miR-342-3p在不同乳腺癌分子亚型及乳腺癌细胞株中的表达  

Expressions of MiR-342-3p in different molecule subtypes and cell lines of breast cancer

作　　者：马涛[1] 吴建中[2] 季明华[3] 何跃君[4] 唐金海[4] 

机构地区：[1]南京医科大学附属无锡妇幼保健院乳腺科,无锡医学博士研究生214002 [2]南京医科大学附属江苏省肿瘤医院中心实验室,南京210000 [3]南京医科大学附属江苏省肿瘤医院放射科,南京210000 [4]南京医科大学附属江苏省肿瘤医院乳腺外科,南京210000

出　　处：《医学研究生学报》2014年第7期718-721,共4页Journal of Medical Postgraduates

基　　金：无锡市卫生局面上指令性项目(ML201307)

摘　　要：目的：乳腺癌的个体化治疗越来越受到人们的重视，不同乳腺癌分子亚型具有不同的预后和治疗方案，研究发现miR-342-3p与乳腺癌激素受体和内分泌治疗耐药相关，与肿瘤细胞凋亡相关，课题进一步研究miR-342-3p在不同乳腺癌分子亚型和不同乳腺癌细胞株中的表达，揭示miR-342-3p在乳腺癌个体化治疗中的重要作用。方法收集乳腺癌手术患者组织标本共90例及培养乳腺癌细胞株MCF-7、SKBr3和MDA-MB-231，使用实时荧光定量PCR检测miR-342-3p在乳腺癌组织中的表达情况。结果 miR-342-3p在不同分子亚型乳腺癌组织中的表达以Lumina B型最高（1．594±0．465），其次为Lumina A 型（1．386±0．443），再次是Her-2高表达型（1．165±0．337），三阴性乳腺癌组织中表达最低（0．837±0．351），差异有统计学意义（P＜0．05）；miR-342-3p的表达在不同年龄组、有无淋巴结转移及与肿瘤大小、组织分级和病理分期方面，差异无统计学意义（P＞0．05）；miR-342-3p在乳腺癌细胞株中的表达，以SKBr3为参照，MCF-7的相对倍数为126（118～134），MDA-MB-231的相对倍数为0．017（0．014～0．018）。结论 miR-342-3p在不同乳腺癌分子亚型和乳腺癌细胞株中表达存在差异，与乳腺癌不同分子亚型相关，可能成为乳腺癌分型的参考指标和好的预后相关。Objective The individualized treatment of breast cancer have attracted more and more attention .Different mo-lecular subtypes of breast cancer have different kinds of prognosis and therapeutic regimen .Studies have found that miR-342-3p is asso-ciated with breast cancer of hormone receptor and endocrine therapy resistance , as well as tumor cell apoptosis .This study was to fur-ther investigate the expressions of miR-342-3p in different breast cancer molecular subtypes and breast cancer cell lines to reveal the importance of miR-342-3p in individualized treatment of breast cancer . Methods A total of 90 tissue samples from patients with breast cancer surgery were collected .Three types of breast cancer cell line were cultured , including MCF-7, SKBr3 and MDA-MB-231.Real-time fluorescent quantitative PCR was applied to detect the expression of miR-342-3p in breast cancer tissue . Results Expression of the miR-342-3p increased the most in Lumina B type breast cancer tissue （1.594 ±0.465）, followed by Lumina A type （1.386 ±0.443）, Her-2 high expression type （1.165 ±0.337）, and the lowest in the tripe negative breast cancer tissue （0.837 ± 0.351）, representing significant difference （P〈0.05）.There was no statistical difference in the expression of the miR-342-3p as to different age groups, lymph node metastasis and tumor size, histological grading and staging （P〉0.05）.As to the expression of miR-342-3p in three types of breast cancer cell line , taking SKBr3 as the reference, the relative ratio was 126（118-134） and MDA-MB-231 was 0.017（0.014-0.018）. Conclusion The expressions of miR-342-3p are different in different molecular subtypes and cell lines of breast cancer , which are relevant to different molecular subtypes of breast cancer , making it possible reference index for breast cancer typing and relevant to good prognosis .

关 键 词：乳腺癌 miR-342—3p 分子亚型 

分 类 号：R737.9[医药卫生—肿瘤]