乙醇激活的鼻咽癌细胞氯电流的分子机制  

Molecular mechanism underlining ethanol-induced chloride currents in nasopharyngeal carcinoma cells

作　　者：林娜[1] 左婉红[1] 赖周毅 伍嘉宝 陈美苑[1] 汪源[1] 陈丽新[2] 王立伟[1] 

机构地区：[1]暨南大学医学院生理学系,广东广州510632 [2]暨南大学医学院药理学系,广东广州510632

出　　处：《中国病理生理杂志》2014年第7期1179-1183,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81272223);广州市科技计划(No.2013J500015);教育部博士点基金资助项目(No.20124401110009);东莞市科技计划(No.2011108102006)

摘　　要：目的：研究乙醇对低分化鼻咽癌CNE-2Z细胞氯通道的影响并探讨其分子机制。方法：MTT检测乙醇对细胞生长的影响；全细胞膜片钳技术记录乙醇对氯电流的影响；应用氯通道阻断剂分析该电流的特性；siR-NA干扰技术分析乙醇敏感氯通道的分子本质。结果：在等渗灌流液中记录到微弱的背景氯电流，随灌流液中乙醇浓度（0．17～170 mmol/L）的升高，所激活的氯电流呈倒U型曲线状，17 mmol/L的乙醇激活电流达到峰值，电流呈较明显的外向优势，能被高渗液及氯通道阻断剂5-nitro-2-（3-phenylpropylamino）benzoic acid（NPPB）所阻断；干扰ClC-3氯通道基因表达后乙醇激活的氯电流明显减小。结论：乙醇可能通过激活CNE-2Z细胞的ClC-3氯通道，诱导氯电流的产生。AIM：To study the effects and mechanisms of ethanol on chloride channels in poorly differentiated nasopharyngeal carcinoma CNE-2Z cells.METHODS：The effect of ethanol on the cell growth was analyzed by MTT as-say.The technique of whole-cell patch-clamp was used to detect the chloride current .The characteristics of the chloride current were analyzed by using the chloride channel blockers .The siRNA technique was used to analyze the molecular basis of the ethanol-sensitive chloride channels .RESULTS： Under isotonic conditions , the background current was weak and stable.Ethanol at concentrations of 0.17~170 mmol/L activated a chloride current in a concentration-dependent manner （an inverted U-shape）, with a maximum effect at the concentration of 17 mmol/L.The currents showed obviously outward rectification and were susceptible to extracellular hypertonicity and the chloride channel blocker , 5-nitro-2-（3-phenylpropyl-amino） benzoic acid （ NPPB） .ClC-3 siRNA obviously decreased the currents activated by ethanol .CONCLUSION：Ex-tracellular ethanol induces chloride currents through activating the ClC-3 chloride channels .

关 键 词：乙醇 鼻咽肿瘤 氯通道 膜片钳术 

分 类 号：R739.63[医药卫生—肿瘤]