大豆黄酮玻璃体腔注射对大鼠视神经损伤的影响  

Effect of Daidzein intravitreal injection on optic nerve injury in rats

作　　者：唐中俊[1] 黄振平[2] 杨文静[3] 邹勇象 蔡季平[1] 

机构地区：[1]第二军医大学长征医院眼科,中国上海市200003 [2]南京军区南京总医院眼科,中国江苏省南京市210000 [3]第二军医大学基础医学部神经生物教研室,中国上海市200003 [4]第二军医大学长征医院神经外科,中国上海市200003

出　　处：《国际眼科杂志》2014年第8期1378-1381,共4页International Eye Science

基　　金：国家自然科学基金项目(No.81200719)~~

摘　　要：目的:大豆黄酮(Daidzein)玻璃体腔内注射是否对视神经急性损伤有保护和修复作用。方法:SD雄性大鼠72只,建立大鼠急性视神经钳夹损伤模型,随机分为正常组、PBS阴性对照组、大豆黄酮治疗组(10μmol/L,100μmol/L,1 000μmol/L)、鼠神经生长因子(mNGF,100ng/mL)阳性对照组;玻璃体内注射3d后处死各组大鼠,用组织病理(HE)、免疫组化检测以及Western Blot分析来观察和评价视网膜形态变化及进行各组间视网膜Caspase-3蛋白和GAP-43蛋白表达的比较。结果:与正常组和阴性对照组相比,不同浓度的大豆黄酮各治疗组和阳性对照组的视网膜形态结构较完整,Caspase-3蛋白的表达相对降低,GAP-43蛋白的表达相对较高,其差异有统计学意义(P<0.05)。结论:大豆黄酮玻璃体腔注射对大鼠急性视神经损伤具有修复和保护作用。AIM： To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages.M ETHODS： After the crush m odels of acute optic nerve w ere set up,72 m ales SD rats w ere divided into 4 groups random ly as com m on group w ithout surgery, FBS negative control group, Daidzein treatm ent group（ 10μ m ol /L,100μ m ol /L,1000μ m ol /L） and positive control group using rats nerve grow th factor（ m NGF, 100 ng /m L）. Three days after interference, all experimental animals were executed.HE staining was used to evaluate morphologic change of the retina,immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase- 3 and GAP- 43 among the groups. RESULTS： Compared with the normal group and negative control group,retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete,the expression of Caspase- 3 protein was relatively lower, the expression of GAP- 43 protein was relatively higher,the differences have statistically significance（P〈0. 05）.CONCLUSION： Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat＇s acute nerve damages.

关 键 词：大豆黄酮 视神经损伤 CASPASE-3 GAP-43 

分 类 号：R774.6[医药卫生—眼科]