MSAP荧光检测初始数据的自动分析研究  

Automatic Processing of the Initial Data of Methylation Level Detected by Fluorescently Labeled MSAP

作　　者：陈秋博 邹智元 李金龙[2] 张竞涛[2] 徐青[2] 

机构地区：[1]北京交通大学软件学院,北京100044 [2]北京交通大学生命科学与生物工程研究院,北京100044

出　　处：《生物技术通讯》2014年第4期527-531,共5页Letters in Biotechnology

基　　金：国家自然科学基金青年基金(31101711);北京交通大学创新训练计划

摘　　要：目的:建立能够实现甲基化敏感扩增多态性(MSAP)荧光检测初始数据自动分析的方法。方法:以17周龄的北京油鸡为实验材料,应用荧光标记的MSAP方法对肌肉组织基因组DNA甲基化水平进行检测,对获得的MSAP荧光检测初始数据通过直接取整法、整区间放置法和半区间放置法等3种计算机处理方法进行分析和比较。结果:对于MSAP荧光检测初始数据的自动转换,半区间放置法与标准的人工转换方法最接近(P>0.05),整区间放置法次之(P>0.05),而直接取整法与标准的人工转换方法差异最大,达到显著水平(P<0.05)。结论:整区间放置法和半区间放置法适用于MSAP荧光检测初始数据的自动分析,建立了基于半区间放置法的在线自动分析软件MSAP Analyst,可实现MSAP荧光检测初始数据的自动分析。Objective： To built a suitable automatic analysis system based on compute to process the initial da-ta of methylation level detected by fluorescence labeled methylation sensitive amplified polymorphism（MSAP）. Methods： With fluorescently labeled MSAP, the DNA methylation levels in muscle tissues from 17-week-old Bei-jing You chicken were detected. The initial data of MSAP were analyzed using manual processing usually as a standard solution, and three automatic processing systems based on compute： direct integral method, whole interval method and half interval method. Results： In the analysis of initial data of methylation level detected by fluores-cence labeled MSAP, no differences was found between manual processing and whole interval method（P〉0.05） or half interval method（P〉0.05）, and the half interval method is more near to manual processing than whole interval method, but significant difference appeared between manual processing and direct integral method（P〈0.05）. Conclu-sion： The whole interval and half interval methods as a substitute for manual processing can be used to realize the automatic analysis of initial data of methylation level detected by fluorescence labeled MSAP, and one new on-line software named MSAP Analyst based on half interval method was built.

关 键 词：甲基化 甲基化敏感扩增多态性 自动分析 荧光检测 北京油鸡 

分 类 号：Q78[生物学—分子生物学]