碘摄入量对TPC-1甲状腺乳头状癌原位裸鼠模型的影响  

Effects of iodine intake on the orthotopic nude mouse model of human papillary thyroid carcinoma cell line TPC-1

作　　者：叶艳[1,2] 赵树君[1,2] 李永梅[1,2] 孙毅娜[1,2] 林来祥[1,2] 阎玉芹[1,2] 陈祖培[1,2] 

机构地区：[1]天津医科大学代谢病医院内分泌所,300070 [2]卫生部激素与发育重点实验室

出　　处：《中华内分泌代谢杂志》2014年第7期558-561,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（81001226）

摘　　要：目的 构建TPC-1甲状腺乳头状癌原位裸鼠模型,观察碘摄入水平对其肿瘤生长及甲状腺功能的影响.方法 甲状腺乳头状癌细胞系TPC-1以2＆#215;105原位接种于裸鼠左侧甲状腺,术后饲以不同含量碘喂养,随机分3个组：低碘组（LI）、适碘组（NI）和高碘组（HI）.在4、12周时处死动物分离甲状腺肿瘤称重,并分别测定血清甲状腺激素水平、尿碘水平,进行肿瘤组织形态学观察.结果 尿碘水平与碘摄入水平相平行表明分组成功,但3组裸鼠甲状腺肿瘤重量的差异无统计学意义（P＞0.05）.4周时NI组甲状腺激素水平正常;LI组T4降低,T3正常;而HI组T4-过性升高,T3下降（P＜0.05）.12周时受原位肿大的甲状腺肿瘤的影响,3组甲状腺激素水平都降低,其中LI组T3、T4下降最为明显,而HI组却表现T3降低,T4正常.结论 碘摄入水平可能不会明显影响TPC-1甲状腺乳头状癌原位裸鼠模型的肿瘤生长,然而它对甲状腺激素的合成有显著影响.Objective To observe the effects of different iodine intake on the thyroid tumor growth and thyroid function in the orthotopic nude mice model of human papillary thyroid carcinoma （PTC） cell line TPC-1.Methods Human PTC cell line TPC-1 （2×10^5） was injected into the left thyroid gland of nude mice.After the operation,the nude mice were randomly divided into three groups：low iodine group （LI）,normal iodine group （NI）,and high iodine group（HI,50 folds of normal iodine） based on the iodine levels contained in their diet.4 and 12 weeks later,the nude mice were executed,then their thyroid tumors were removed and weighted.The levels of urinary iodine were measured with As^3＋-Ce^4＋ catalytic spectrophotometry using ammonium persulfate digestion method.The thyroid hormone level was detected using chemiluminescent immunoassay.The morphology and structure of thyroid tumor tissue was observed by microscope.Results The iodine intervention feeding was successful according to urinary iodine level of LI,NI,and HI groups,paralleled to their iodine intakes.However,the difference of the weight of thyroid tumor in three groups had no statistical significance（P〉0.05）.At 4 weeks,compared with control group,the levels of thyroid hormones were normal in NI group,while lower T4 and normal T3 were found in LI group.However,T4 was higher and T3 was lower in HI group （P〈0.05）.At 12 weeks,the levels of thyroid hormones all were decreased due to the enlargement of thyroid gland tumor in NI,LI and HI groups.T4 and T3 in LI group were the lowest among three groups,even T4 was below detection limit.T4 was normal and T3 was lower in HI group as compared to control group.Conclusion Iodine intake may not significantly affect tumor growth in the orthotopic nude mice model of human PTC cell line TPC-1,but it has a significant effect on the synthesis of thyroid hormones.

关 键 词：碘 甲状腺乳头状癌 原位模型 

分 类 号：R736.1[医药卫生—肿瘤]