微波消融联合树突状细胞瘤苗免疫治疗小鼠乳腺癌的疗效及免疫学机制探讨  

The effect of microwave ablation combined with dendritic cell tumor vaccine treatment on mouse mammarycarcinoma and its immunological mechanism

作　　者：周涯[1] 任社华[1] 李永菊[2] 张明[1] 周洪练[1] 

机构地区：[1]贵州省遵义医学院医学物理学教研室,遵义563000 [2]医学免疫学教研室

出　　处：《中华物理医学与康复杂志》2014年第2期91-94,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：贵州省卫生厅项目（D-348）,遵义市红花岗区科技项目（F-297）

摘　　要：目的 观察微波消融联合树突状细胞（DC）瘤苗免疫治疗小鼠乳腺癌的疗效并探讨其免疫学机制.方法 采用随机数字表法将48只BALB/c小鼠分为模型组、微波消融组、DC瘤苗免疫组及联合治疗组.将上述小鼠制成乳腺癌实验动物模型,微波消融组于荷瘤14d时给予微波消融治疗,DC瘤苗免疫组于荷瘤7d时给予DC瘤苗免疫,联合治疗组分别于上述时间点给予DC瘤苗免疫及微波消融治疗.观察各组小鼠存活、肿瘤生长及肺转移情况；于微波消融处理完后第14天时采用流式细胞术检测各组小鼠脾细胞中CD3＋、CD4＋ T细胞比例变化；采用酶联免疫吸附法（ELISA法）检测各组小鼠血清干扰素-γ（IFN-γ）和白介素-4（IL-4）水平.结果 联合治疗组小鼠存活时间[（56.4±4.3）d]、肿瘤面积[（67.3±6.1）mm2]及肺转移指数（0.6±0.4）均明显优于微波消融组及DC瘤苗免疫组（P＜0.05）；联合治疗组脾细胞中CD3＋T细胞比例[（27.8±10.3）％]、CD4＋T细胞比例[（16.7±8.3）％]均较微波消融组及DC瘤苗免疫组明显增加（P＜0.05）；另外联合治疗组血清中IFN-γ浓度[（41.8±22.9）pg/ml]较微波消融组及DC瘤苗免疫组显著升高（P＜0.05）,而IL-4浓度[（15.4±6.4） pg/ml]则较微波消融组及DC瘤苗免疫组明显降低（P＜0.05）.结论 联合采用微波消融及DC瘤苗免疫治疗乳腺癌小鼠具有协同作用,与单纯微波消融或DC瘤苗免疫比较,该联合疗法能进一步抑制实验小鼠乳腺癌肿瘤生长,其治疗机制可能与增强小鼠免疫反应有关.Objective To investigate the therapeutic effects of microwave ablation combined with dendritic cell （DC） tumor vaccine on a murine breast cancer model and to explore its immunological mechanism.Methods Forty-eight Balb/c mice were used to establish models of mouse mammary carcinoma,and then randomly divided into a sham-operation group,a microwave ablation treated group,a DC tumor vaccinated group and a microwave ablation plus DC tumor vaccinated group.Microwave ablation was conducted with the microwave ablation group on day 14 after establishing the model.DC tumor vaccination was performed on day 7 in the vaccinated group.In the microwave ablation plus DC tumor vaccinated group,DC tumor vaccination and microwave ablation were consecutively performed on days 7 and 14,respectively.Tumor growth,lung metastasis and survival were observed.The proportion of CD3＋ and CD4＋ T cells in splenocytes were analyzed by fluorescence Activated Cell Sorter （FACS） on the 14th day after microwave ablation.Serum levels of interferon-γ （IFN-γ） and interleukin-4 （IL-4） were also detected using ELISA.Results The survival time （56.4 ± 4.3 days）,tumor size （67.3 ± 6.1 mm2） and lung metastasis index （0.6 ± 0.4） in the microwave ablation plus DC tumor vaccinated group were all significantly lower than the corresponding averages in the microwave ablation and DC tumor vaccinated group.The proportions of splenic CD3 ＋ T cells （27.8 ±10.3 %） and CD4＋ T cells （16.7 ± 8.3 %） in the microwave ablation plus DC tumor vaccinated group were significantly larger than in the control group.Average serum levels of IFN-γ （41.8 ± 22.9 pg/ml） in the microwave ablation plus DC tumor vaccinated group were also significantly larger than in the control group,while the average serum level of IL-4 （15.4±6.4 pg/ml） was significantly lower.Conclusions Microwave ablation and DC tumor vaccination together could have synergistic effects in treating experimental breast cancer,which might be closely related to

关 键 词：乳腺癌 微波消融 树突状细胞瘤苗 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]