人胎球蛋白AHSG及补体C3在胰腺癌组织中的表达  

Expression and clinical significance of AHSG and complement C3 in pancreatic ductal adenocarcinoma

作　　者：陈炯[1] 武文[1] 陈龙江[1] 马小磊[1] 赵跃[1] 周杭城[1] 杨仁保[1] 胡立威[1] 

机构地区：[1]安徽医科大学附属省立医院普外科,合肥230001

出　　处：《中华医学杂志》2014年第28期2175-2179,共5页National Medical Journal of China

基　　金：国家自然科学基金（81071985）;安徽省科技攻关计划项目（07010302203;11010402172）;安徽省国际科技合作计划项目（1008070038）

摘　　要：目的 比较胰腺癌患者、胰腺良性肿瘤患者、慢性胰腺炎患者和正常对照组人群血清蛋白表达的差异,寻找胰腺癌潜在的特异性肿瘤标志物.方法 利用双向差异凝胶电泳（2D-DIGE）联合基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）技术分离并鉴定2009年5月至201 1年4月40例胰腺癌患者、10例良性肿瘤患者、10例慢性胰腺炎患者和40例正常对照组人群外周血清蛋白质.选取已鉴定出的胰腺癌差异表达蛋白中未见报道的新蛋白或差异性较显著的1～2种蛋白,利用荧光实时定量-聚合酶链反应（Real time PCR）、Western印迹和免疫组化方法分别从转录水平和蛋白水平进行验证.结果 初步筛选并鉴定出12个在胰腺癌组存在差异表达的蛋白,包括补体C3（C3）、血色素结合蛋白、人胎球蛋白（AHSG）、载脂蛋白H、转铁蛋白、结合珠蛋白、载脂蛋白E、转甲状腺素蛋白、血清淀粉样蛋白P成分、玻连蛋白、凝血素.Real-time PCR结果显示C3、AHSG在胰腺癌组织中的表达水平显著高于正常胰腺组织;而Western印迹结果同样显示胰腺癌组中C3、AHSG的表达量显著高于正常胰腺组,胰腺癌组C3、AHSG灰度比分别为0.21 ±0.01、0.78±0.03,正常胰腺组C3、AHSG灰度比分别为0.11±0.01、0.26±0.02;免疫组化结果表明C3、AHSG主要表达部位为胞质,C3、AHSG在胰腺癌组织当中的阳性表达率分别为72.5％、82.5％,而在正常对照组则分别为32.5％、25％.结论 补体C3和AHSG在胰腺癌中的高表达具有普遍性,可能是潜在的胰腺癌血清标志物.Objective To analyze serum proteins from pancreatic carcinoma patients,pancreatic benign tumor patients,chronic pancreatitis patients and normal controls to discover potential and specific biomarkers.Methods Serum samples were collected from 40 pancreatic carcinoma patients,10 pancreatic benign tumor patients,10 chronic pancreatitis patients and 40 cancer-free controls from May 2009 to April 2011.The samples were compared with two-dimensional differential gel electrophoresis （2D-DIGE） and differentially expressed proteins were further identified by matrix-assisted laser desorptiorn/ionization time of flight mass spectrometry （MALDI-TOF-MS）.Then,two up-regulated proteins were further validated by realtime polymerase chain reaction （PCR）,Western blot analysis and immunohistochemistry （IHC） from transcriptional and proteinic aspects.Results We identified 12 differently expressed proteins in pancreatic carcinoma group compared with normal control group,including complement component C3,hemopexin,alpha-2-HS-glycoprotein,apolipoprotein H,serotransferrin,haptoglobin,apolipoprotein E,transthyretin,serum amyloid P-component,vitronectin,proothrombin and isoform 2 of Ig mu chain C region.High level of C3 and AHSG were detected in cancerous tissues by real-time PCR,Western blot and immunohistochemisty.Western blot revealed that gray ratios of C3 and AHSG were 0.11 ±0.01 and 0.26 ±0.02 respectively.TheImmunohistochemical results showed that positive rate of C3 and AHSG were 72.5％ and 82.5％ in cancerous group versus 32.5％ and 25％ respectively in normal control.Conclusion C3 and AHSG may become pancreatic carcinoma-related biomarkers.

关 键 词：胰腺肿瘤 诊断 生物标志物 蛋白质组学 

分 类 号：R735.9[医药卫生—肿瘤]