基于HLA等位基因频率的全球人群亲缘关系初探  

Primary analysis on affinity of worldwide populations using allele frequency of HLA

作　　者：黄庆海[1] 吴亚萌 李震[1] 谢维[1] 

机构地区：[1]"发育与疾病相关基因"教育部重点实验室东南大学生命科学研究院,江苏南京210096

出　　处：《东南大学学报（医学版）》2014年第4期429-435,共7页Journal of Southeast University(Medical Science Edition)

基　　金：国家杰出青年基金资助项目(30325017)

摘　　要：目的:探讨不同实验方法、不同统计方法的HLA配型数据在分析全球人群亲缘关系中的作用和效能,为运用HLA基因的多态性分析全球人群的遗传关系提供理论依据。方法:收集了全球6大洲10个地理分区中的110个人群HLA-A,-B和-DRB1座位的高分辨率配型数据,并对这些等位基因命名进行了归一化处理,用统一的统计方法重新统计了各人群的等位基因频率,用N-J法构建了全球人群的关系树,进而对全球人群间的亲缘关系进行了初步分析。结果:同一地理区域内的人群相互聚类,不同区域间的人群完全分离。关系树不仅能很好地分析局部地区相似人群的异同,也能有效地分析全球各区域人群间的亲缘关系。结论:HLA基因的多态性适合对全球人群的亲缘关系进行研究,也是一种高效的研究人类学的分子标记物。Objective： To explore whether HLA can be applied to construct phylogenetic trees to analyze affinity of worldwide populations, and provide a reference for further analyzing relationship among populations regionally and globally using HLA allele frequency. Methods： High-resolution typing data for HLA-A,-B and-DRBI from 112 healthy populations in 10 geographic regions spanning six continents were compiled from published literatures. Primary analyses on affinity of global populations were performed according neighbor-joining trees constructed by allele frequency of HLA. Results： Worldwide populations from the same and different geographic regions were grouped and separated, respectively. Populations within a close geographic region were clustered into a clade. Conclusion： Phylogenetic analysis could clearly classify populations from the six continents, and polymorphism of HLA is a useful tool in anthropological studies.

关 键 词：人类白细胞抗原 等位基因频率 免疫遗传学 群体遗传学 

分 类 号：R442.8[医药卫生—诊断学]