内毒素诱导小鼠葡萄膜炎视网膜中补体系统的变化  

Alternation of retinal complement system in a mouse model of lipopolysaccharide-induced uveitis

作　　者：陶雪莹[1] 郑仕洁 雷博[1] 

机构地区：[1]重庆医科大学附属第一医院眼科//重庆市眼科学重点实验室,重庆400016

出　　处：《南方医科大学学报》2014年第8期1121-1124,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81271033)~~

摘　　要：目的研究内毒素诱导葡萄膜炎(EIU)视网膜中补体系统的活化情况。方法将Balb/c小鼠随机分为对照组和实验组,通过玻璃体腔注射内毒素建立EIU小鼠模型。建模24 h后,分离对照组和实验组小鼠的视网膜,分别提取总蛋白和总mRNA。采用实时荧光定量PCR检测视网膜中补体经典途径(C1qb、C4b)、血凝素途径(MASP1、MASP2)和替代途径(CFB、C3)、末端通路(C5)中关键因子mRNA的表达水平。采用Western blotting检测视网膜中补体系统经典途径和替代途径中的关键因子(C1q、C4、C3)蛋白的表达情况。结果正常小鼠视网膜细胞可表达大部分补体系统成分的基因。同对照组相比,实验组小鼠视网膜中C1qb、C4b、CFB、C3的mRNA表达水平升高;MASP1、MASP2在视网膜上表达量很低,实验组与对照组无统计学差异。同时实验组C1q、C4、C3的蛋白水平比对照组也明显升高。结论 EIU小鼠在炎症高峰期补体系统尤其经典途径和替代途径被激活,激活的补体系统可能在EIU的病理过程中起作用。Objective To investigate the activation of the complement system in the retina in a mouse model of endotoxin-induced uveitis （EIU）. Methods Balb/c mice were randomly divided into control group and lipopolysaccharide （LPS）-induced EIU group. Twenty-four hours after modeling, the expressions of the major complement components of the classical pathway （CP）, mannose-binding lectin （MBL） pathway, alternative pathway （AP）, and terminal pathway in the retina were determined by quantitative RT-PCR. Western blotting was employed to examine the protein expressions of the key components of the complement system involved in the CP and AP pathways in the retina. Results Normal mouse retina expressed a variety of complement components that were involved mainly in the CP and AP pathways. The expressions of the complement components involved in the CP and AP pathways were up-regulated in the retina of mice with EIU. Although MASP1 and MASP2 were detected in the retina in both the EIU and control groups, their expressions were weak and showed no significant difference between the two groups. Conclusion The AP and CP but not the MBL pathways of the complement system are activated in the retina of mice with EIU, suggesting a role of the activated complement system in the pathological vrocess of EIU.

关 键 词：内毒素诱导葡萄膜炎 补体活化 经典途径 替代途径 视网膜 

分 类 号：R773.9[医药卫生—眼科]