食管鳞癌中14-3-3σ表达的临床意义及与预后的相关性  

Clinical relevance and correlation with prognosis of 14-3-3σ expression in esophageal squamous cell carcinoma

作　　者：魏华[1,2] 王明[1] 刘瑞敏[1] 张天[1] 马瑾[1] 马远方[1] 齐义军[1] 

机构地区：[1]河南大学医学院细胞与分子免疫学重点实验室,河南开封475004 [2]河南大学淮河医院内窥镜室,河南开封475004

出　　处：《基础医学与临床》2014年第8期1044-1048,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81072039)

摘　　要：目的 确定14-3-3σ与食管鳞状细胞癌（ESCC）临床病理特征及与预后的相关性.方法-86℃冻存的TNMⅠ～Ⅳ期ESCC 80例,ESCC组织芯片含ESCC 86例;分别应用Western blot、免疫组织化学检测80例冻存、组织芯片中86例ESCC中14-3-3σ的表达;Kaplan-Meier分析14-3-3σ、临床病理资料与ESCC术后整体生存期相关性.结果 14-3-3σ蛋白主要表达于胞质及胞膜,其阳性表达与淋巴结转移、TNM分期呈负相关,与分化程度正相关（P＜0.05）;14-3-3σ高表达及低表达ESCC患者术后平均生存时间分别为39.82和28.45个月,5年整体生存率分别为43.31％和24.8％（log-rank test,P＜0.01）,TNM分期和14-3-3σ是影响ESCC预后的独立因素.结论 14-3-3σ低/失表达可能是ESCC预后不良的分子标志物之一.Objective This study aims its prognostic value in ESCC. Methods to determine the correlation of 14-3-3σ expression with clinical features and Eighty frozen ESCC samples of TNM Ⅰ to Ⅳ stage stored at -86 ℃ and a tissue microarray containing 86 fixed ESCC samples were used. Western blot was used to examine the 14-3-3σ protein expression in frozen ESCC, and IHC to estimate the 14-3-3σ protein expression in 86 ESCC samples from tissue microarray. The association of 14-3-3σ protein expression with clinical features and ESCC prognosis was de- termined by Kaplan-Meier curves. Results 14-3-3σ immunostaining was located predominantly in the cytoplasm and plasma membrane. 14-3-3σ was positively correlated with differentiation, and negatively with lymph node metastasis and TNM stage（P 〈 0. 05 ）. The average survival time of ESCC patients with 14-3-3σ high and low expression was 39.82 months and 28.45 months, respectively, and the overall 5 year survival rate were 43.31% and 24. 8%, respectively （log-rank test, P 〈 0. 01 ）. TNM stage and 14-3-3σ were independent prognostic factors of ESCC. Conclusions Low/loss protein expression of 14-3-3σ may correlate with poor prognosis of ESCC.

关 键 词：食管鳞状细胞癌 14-3-3σ 生物标志物 预后 

分 类 号：R735.1[医药卫生—肿瘤]