核心蛋白多糖抑制翼状胬肉成纤维细胞增生的实验研究  

Inhibitive effects of Decorin on proliferation of cultured human pterygium fibroblasts

作　　者：张海江[1] 霍鸣[1] 董洁玉[1] 宋庆磊[1] 

机构地区：[1]三峡大学第一临床医学院宜昌市中心人民医院眼科,湖北省宜昌市443003

出　　处：《眼科新进展》2014年第8期725-728,共4页Recent Advances in Ophthalmology

摘　　要：目的观察核心蛋白多糖对体外培养人翼状胬肉成纤维细胞（human pterygium fibroblasts,HPF）增生和凋亡的影响,寻找辅助治疗和预防翼状胬肉切除术后复发的新方法。方法采用组织块法体外培养HPF,将处于对数生长期的细胞用于实验。将0.1 mg·L-1、1.0 mg·L-1、10.0 mg·L-1的Decorin分别加入细胞培养基中培养24 h、48 h、72 h。MTT比色法分别测定不同浓度的Decorin作用于HPF不同时间后的细胞生长抑制率,流式细胞仪测定细胞周期时相变化,免疫组织化学法染色PCNA检测细胞生长活性。结果 MTT结果显示不同浓度组Decorin作用HPF 24 h细胞生长抑制率分别为（7.81±1.26）%、（19.52±2.14）%、（42.69±1.62）%,作用48 h分别为（10.19±2.74）%、（22.28±0.97）%、（45.73±0.82）%,作用72 h分别为（20.49±1.39）%、（29.50±2.47）%、（59.41±1.71）%。随着药物浓度的逐渐增高和作用时间的延长,细胞生长抑制率逐渐增加,差异均有统计学意义（均为P〈0.05）。流式细胞仪检测各浓度Decorin组HPF中G0/G1期细胞比率均较空白对照组高,差异均有统计学意义（均为P〈0.05）。当Decorin浓度在0.1~10.0 mg·L-1范围内作用HPF 72 h,均能够明显抑制细胞表达PCNA（均为P〈0.05）。结论 Decorin可以抑制HPF的增生,诱导其凋亡,且呈明显的剂量和时间依赖性,有望成为防治翼状胬肉的药物之一。Objective To observe the effects of Decorin on proliferation and apoptosis of human pterygium fibroblasts（ HPF） cultured in vitro, and offer new options to treat pterygium and prevent its recurrence rate after surgery. Methods HPF was cultured in vitro and treated by 0. 1mg·L- 1,1. 0 mg·L- 1, 10. 0 mg·L- 1 Decorin for 24 hours, 48 hours, 72 hours, respectively.The MTT method was used to assay the inhibitive ratio of cell growth at different doses and time points. The cell growth cycle was checked with flow cytometry. The expression of proliferation cell nuclear antigen（ PCNA） in each group was detected by immunohistochemistry method. Results According to MTT assay,the ratio at 24 hours were（ 7. 81 ± 1. 26） %,（ 19. 52 ± 2. 14） %,（ 42. 69 ± 1. 62） %, respectively, 48 hours were（ 10. 19 ± 2. 74） %,（ 22. 28 ± 0. 97） %,（ 45. 73 ±0. 82） %, respectively, 72 hours were（ 20. 49 ± 1. 39） %,（ 29. 50 ± 2. 47） %,（ 59. 41 ± 1. 71） %,respectively. The inhibitive ratio of cell growth in all Decorin groups were promoted after three time points, and there was a concentration- and time-dependence（ all P ＆lt; 0. 05）. The results of flow cytometry showed that the percentages of HPF in G0 /G1 phase were increased in Decorin groups compared with blank group（ all P ＆lt; 0. 05）. Within 0. 1- 10. 0 mg·L- 1 Decorin about 72 hours, it could obvious inhibit the expression of PCNA in HPF（ all P ＆lt; 0. 05）. Conclusion Decorin can effectively inhibit the growth of HPF and induce their apoptosis in a dose-and timedependent manner,which is expected to become the drug for treatment and prevent of pterygium.

关 键 词：核心蛋白多糖 翼状胬肉 成纤维细胞 转化生长因子 

分 类 号：R733.7[医药卫生—肿瘤]