松果菊苷对帕金森病大鼠纹状体及海马细胞外液中单胺类神经递质的影响  

Effects of echinacoside on striatal and hippocampus extracellular fluid of monoamine neurotransmitter in Parkinson's disease rats

作　　者：张万鑫[1] 马婧怡[1] 陈虹[1] 姜勇[2] 屠鹏飞[2] 丁慧[1] 

机构地区：[1]石河子大学药学院教育部新疆特种植物药资源重点实验室,新疆石河子832002 [2]北京大学中医药现代研究中心,北京100083

出　　处：《中国药理学通报》2014年第8期1131-1136,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30860334;30560171)

摘　　要：目的研究松果菊苷(echinacoside,ECH)对帕金森病(Parkinson's disease,PD)大鼠纹状体及海马细胞外液中单胺类神经递质的影响,以探讨ECH对脑神经保护作用的可能机制。方法采用双点注射6-羟基多巴胺损毁术制作PD模型,各组大鼠腹腔注射相应的药物或生理盐水连续4周,进行双靶点微透析程序,将透析液注入高效液相-电化学检测器(HPLC-ECD),测定各组大鼠纹状体及海马细胞外液中多巴胺(DA)、3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)、去甲肾上腺素(NE)及5-羟色胺(5-HT)的含量。结果与对照组相比,模型组大鼠纹状体及海马细胞外液中NE、DA、DOPAC、HVA、5-HT含量均明显降低(P<0.01);与模型组相比,各给药组大鼠的纹状体及海马细胞外液中5种物质的含量均明显升高(P<0.05,P<0.01);ECH高剂量组与阳性药MD组相差不大。结论 ECH对PD大鼠纹状体及海马细胞外液中的单胺类神经递质的含量减少具有改善作用,对于PD具有一定的治疗作用,这是ECH对PD大鼠脑神经保护作用的可能机制之一。Aim Tostudytheeffectsofechinacoside on striatal and hippocampus extracellular fluid of mono-amine neurotransmitter in Parkinsonˊs disease rat, and discuss the possible mechanism of the ECH effect on brainprotection.Methods Doublepointinjectionof 6-hydroxy dopamine damaged was adopted to make PD model, the corresponding drugs or normal saline were intraperitoneally injected for 4 consecutive weeks, mi-cro-dialysis program in double targets was taken after treatment, the dialysate was injected into HPLC-EDC, and striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NA, 5-HT levels of each group were measured. Results Compared with control group, striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NE, 5-HT levels were significantly re-duced in model group （ P 〈0. 01 ）; compared with model group, striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NE, 5-HT levels were significantly increased in treatment group （ P 〈0 . 05 , P〈0. 01 ） , and the content of five kinds of substances in ECH high dose group was similar to that in MD group.Conclusion ECHcouldincreaseextracellular monoamine neurotransmitters in striatum and hippo-campus, ECH has a therapeutic effect on PD, and echinacoside is possible mechanisms of the brain pro-tective effect of PD rats.

关 键 词：松果菊苷 帕金森病 纹状体 海马 微透析 单胺类神经递质 

分 类 号：R-332[医药卫生]