血清TNF-α升高对糖尿病肾脏脂质积聚的影响研究  

Effect of increased serum TNF-α on lipid accumulation in kidney of diabetes

作　　者：张松[1] 朱琳[2] 郝军[3] 郑书深[3] 

机构地区：[1]河北医科大学第三医院检验科,河北石家庄050051 [2]河北医科大学第三医院肌电图室,河北石家庄050051 [3]河北医科大学基础医学院病理学教研室,河北石家庄050017

出　　处：《中国药理学通报》2014年第8期1161-1165,共5页Chinese Pharmacological Bulletin

基　　金：河北省科技支撑计划项目(No 13277740D)

摘　　要：目的明确糖尿病小鼠血清TNF-α升高与肾脏脂质代谢的关系。方法 CD1小鼠按体重腹腔注射链脲佐菌素(150 mg·kg-1),72 h后空腹血糖大于16.7 mmol·L-1者确定为1型糖尿病模型,饲养1个月后,采用ELISA方法检测血清中TNF-α水平,免疫组织化学和Western blot方法检测SREBP-1和ADRP的表达。结果与正常对照小鼠相比,糖尿病小鼠血清TNF-α水平明显升高,为正常对照小鼠的7.73倍。SREBP-1和ADRP主要表达于肾小管上皮细胞,糖尿病小鼠肾脏SREBP-1前体片段、成熟片段和ADRP表达明显高于正常对照小鼠,分别是对照组的2.31倍、1.74倍和1.72倍。小鼠血清TNF-α水平和肾脏ADRP表达的相关性分析证实二者间存在正相关,相关系数为0.914。结论糖尿病小鼠血清TNF-α升高可能是导致肾脏脂质积聚的因素之一。Aim To determine the relationship between serum TNF-α and renal abnormal lipid metabolism in diabetic mice. Methods CD1 mice were injected intraperitoneally with STZ （150 mg· kg-1） and the type 1 diabetic mice were determined with high fasting blood glucose （ 〉 16.7 mmol·L-1） 72 hours after injection. After fed for one month, normal control mice and diabetic mice were sacrificed. The serum TNF-α level was detected by the method of ELISA. Immunohistochemistry and Western blot were used toexplore the expression of SREBP-1 and ADRP. Resuits The results of ELISA showed that serum TNF-α was increased by 7.73 times in diabetic mice compared with normal mice. SREBP-1 and ADRP expressions were located in renal tubular cells. Again, it was con firmed by Western blot that SREBP-1 precursor segment, SREBP-1 mature segment and ADRP were re spectively enhanced by 2.31 times, 1.74 times and 1. 72 times in diabetic mice in comparison with normal control mice . In addition , the data analysis revealed apositive correlation （correlation coefficient 0. 914） between serum TNF-ct and renal ADRP expression. Conclusion The increased serum TNF-α may be one of factors involved in renal lipid accumulation of diabetes.

关 键 词：肿瘤坏死因子A 糖尿病 肾小管上皮细胞 脂质积聚 脂肪分化相关蛋白 固醇调节元件结合蛋白-1 

分 类 号：R-332[医药卫生]