水通道蛋白9对肝癌细胞迁移能力的影响  

Effect of aquaporin-9 expression on migratory ability of liver cancer cells

作　　者：郑玉杰[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆外科,湖南长沙410011

出　　处：《中国普通外科杂志》2014年第7期921-925,共5页China Journal of General Surgery

基　　金：湖南省自然科学基金资助项目(10JJ3055)

摘　　要：目的:通过油酸诱导肝癌HepG2细胞水通道蛋白9(AQP9)的表达观察后者对HepG2细胞株迁移能力的影响。方法:HepG2细胞经不同处理后(0、250、500μmol/L油酸处理或AgNO3+500μmol/L油酸处理),用Western blot法检测AQP9蛋白的表达,细胞划痕实验检测细胞迁移能力。结果:经油酸处理后,HepG2细胞AQP9蛋白表达增加,细胞迁移能力增强,且呈浓度依赖性(均P<0.05),油酸的上述作用被预先给予水通道蛋白抑制剂AgNO3所取消。结论:肝癌HepG2细胞的迁移能力与其AQP9表达有关,AQP9表达越高细胞的侵袭力越强。Objective： To observe the influence of aquaporin 9 （AQP9） on migratory ability in liver cancer HepG2 cells through induction of its expression by oleic acid. Methods： In HepG2 cells following different treatment （treatment of 0, 250, and 500 μmol/L oleic acid, or treatment of AgNO3＋500 μmol/L oleic acid）, the AQP9 protein expression was determined by Western blot analysis and cellular migratory ability was measured by scratch assay, respectively. Results： After oleic acid treatment, the AQP9 protein expression was significantly up-regulated in HepG2 cells, and their migratory ability was significantly increased, which all presented a concentration-dependent manner （all P＆lt;0.05）, while, these effects of oleic acid were abolished by pretreatment of the aquaporin inhibitor AgNO3. Conclusion： The migratory ability of liver cancer HepG2 cells is associated with the AQP9 expression, which is the higher the AQP9 expression, the stronger the migratory ability of the HepG2 cells.

关 键 词：癌 肝细胞 水通道蛋白质9 细胞运动 

分 类 号：R735.7[医药卫生—肿瘤]