高三尖杉酯碱对急性单核细胞白血病SHI-1细胞株诱导凋亡机制的研究  

Inducing-apoptosis effect of homoharringtonine on acute monocytic leukemia cell line SHI-1 and its relevant mechanism

作　　者：赵雨辰[1] 杨明珍[1] 

机构地区：[1]安徽医科大学第一附属医院血液内科,合肥230022

出　　处：《安徽医科大学学报》2014年第8期1080-1084,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高等学校省级自然科学研究重点项目(编号:KJ2011A164)

摘　　要：目的研究高三尖杉酯碱(HHT)作用于急性单核细胞白血病(AML-M5)SHI-1细胞株的机制,是否可抑制SHI-1细胞的增殖、诱导细胞凋亡;了解HHT对AML-M5 SHI-1细胞膜电位的影响,即其诱导的细胞凋亡是否通过线粒体介导的细胞凋亡途径实现。方法将不同浓度(5、10、50、100、500μg/L)的HHT作用SHI-1细胞,瑞氏染色观察细胞镜下形态学改变、流式细胞术AnnexinV-FITC/PI法检测细胞凋亡、CCK-8法测细胞增殖抑制作用、线粒体膜电位试剂盒(线粒体染料JC-1活细胞染色)检测线粒体跨膜膜电位(Δψm)变化。结果 HHT能有效抑制SHI-1细胞的增殖、诱导其凋亡,随着药物浓度的增高,细胞凋亡率、增殖抑制率明显增加(P<0.05)。流式细胞仪线粒体膜电位结果表明,HHT作用后存在明显荧光变化,细胞线粒体膜电位下降,并呈浓度相关性。结论 HHT可抑制AML-M5 SHI-1细胞株的增殖、诱导其凋亡,其诱导细胞凋亡可能是通过线粒体介导的细胞凋亡途径实现。Objective To explore the effect of homoharringtonine （HHT） on proliferation and induce apoptosis of acute monocytic leukemia cell line SHI-1,and whether the induction of apoptosis is mediated by mitochondrial apoptosis pathway.Methods Experiments were divided into control and test groups （5,10,50,100,500 μg/L）.The growth inhibitory rate of SHI-1 cells was detected using Cell Counting Kit-8 （CCK-8） method,while the cell apoptotic rate was measured by AnnexinV-FITC/PI using flow cytometry.Mitochondrial membrane potential across the membrane（△ψm） was determined by mitochondrial membrane potential assay kit with JC-1.Results HHT inhibited the growth of SHI-1 cells and induced apoptosis in concentration-dependent manners （P ＜ 0.05）.Mitochondrial membrane potential across the membrane analysis showed that SHI-1 cells significantly showed an alteration of fluoresence after treating with HHT for 20 hours,as compared with control group.Conclusion HHT is able to inhibit proliferation and induce apoptosis of SHI-1 cells,the mechanism may be associated with the mediation by mitochondrial apoptosis pathway.

关 键 词：高三尖杉酯碱 SHI-1细胞 急性单核细胞白血病 细胞增殖 细胞凋亡 

分 类 号：R733.71[医药卫生—肿瘤] R979.19[医药卫生—临床医学]