紫杉醇增强鼻咽癌细胞放射敏感性的初步机制研究  

Preliminary mechanism of paclitaxel enhanced radiation sensitivity for nasopharyngeal carcinoma cells

作　　者：陈曦[1] 邹枕玮[2] 潘肖芬 牟晶晶[2] 彭纲[2] 

机构地区：[1]黄石市中心医院肿瘤内一科,湖北黄石435001 [2]华中科技大学同济医学院附属协和医院肿瘤中心

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第15期1129-1132,1136,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：湖北省自然科学基金面上项目资助(No:2012FFB02324)

摘　　要：目的:评价紫杉醇对放射抗拒鼻咽癌细胞的放射增敏及其机制的初步研究。方法:通过X射线大剂量低分割照射技术建立鼻咽癌放射抗拒细胞亚系(子代,CNE-2S1);用紫杉醇单独处理和联合放疗分别处理CNE-2S1,以单纯放疗处理组为对照组。通过细胞克隆形成实验观察紫杉醇对放疗的增敏作用;采用流式细胞仪分析不同处理组CNE-2S1的细胞周期分布及凋亡发生率;免疫印迹分析不同处理组SHP-1表达水平。结果:成功建立鼻咽癌放射抗拒细胞亚系CNE-2S1;细胞克隆实验结果显示紫杉醇对放疗具有明显增敏作用;流式检查结果表明紫杉醇处理后的CNE-2S1处于G2-M期细胞比例明显增高,紫杉醇联合放疗处理组可明显提高CNE-2S1细胞的凋亡比率;免疫印迹结果显示紫杉醇及联合放疗组可降低CNE-2S1细胞中SHP-1的表达水平。结论:紫杉醇可增强放疗对鼻咽癌放射抗拒细胞的作用,SHP-1下调可能参与其中。Objective：To study the radiation-sensitizing funciton and preliminary mechanism of paclitaxel in radiation-resistant nasopharyngeal carcinoma cells.Method：X-ray dose fractionated irradiation technology to build radiation-resistant subline of nasopharyngeal carcinoma;CNE-2S1was treated with paclitaxel alone or combined with radiation therapy,while control group treated with radiation therapy;cell colony formation assay was used to observe sensitizing effect of paclitaxel on radiotherapy;flow cytometry analysis was used to analyze cell cycle distribution and apoptosis ratio of different treatment groups;immunoblotting was used to analyze SHP-1expression levels of different treatment groups.Result：Nasopharyngeal carcinoma cells resistant to radiation was successfully established;cell colony formation assay showed that paclitaxel has obvious sensitizing effect on radiotherapy;FACS results showed that：CNE-2S1treated by paclitaxel were arrested in G2Mphase;paclitaxel and radiotherapy treatments significantly improved the CNE-2S1apoptosis ratio;Western blot results showed that paclitaxel and combined radiotherapy can reduce the CNE-2S1cells SHP-1expression levels.Conclusion：Paclitaxel enhanced radiation therapy for nasopharyngeal carcinoma cells resistant to radiation,and SHP-1may be involved in this progress.

关 键 词：鼻咽肿瘤 紫杉醇 SHP-1 放疗增敏 

分 类 号：R739.63[医药卫生—肿瘤]