DC与同源CIK细胞抗急性粒系白血病细胞作用的研究  

Effect of CIK cells co- cultured with DC cells on acute granulocyte leukemia cells

作　　者：李玲[1] 刘文康[1] 任健康[1] 韩秀蕊[1] 杨娣娣[1] 施瑞洁[1] 王俊燕[2] 魏绪仓[1] 

机构地区：[1]陕西省人民医院,陕西西安710068 [2]第四军医大学唐都医院放免中心,陕西西安710038

出　　处：《现代肿瘤医学》2014年第8期1779-1781,共3页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:2011JM4009)

摘　　要：目的:探讨急性白血病细胞免疫治疗的新途径。方法:自正常健康人外周血分离淋巴细胞进行培养,于不同时间分别加入相应的细胞因子,培养9天后收获CIK和DC细胞。按照1∶5的比例将DC细胞与CIK细胞混合培养,收集共培养的细胞,以不同比例与急性粒系白血病细胞共培养测定细胞杀伤活性。结果:DC细胞与CIK细胞共培养后,其细胞增殖能力、细胞毒活性与单独CIK细胞相比有统计学差异。在同一效靶比对急性粒系白血病细胞均有杀伤作用,随着效靶比的增高对急性粒系白血病细胞的杀伤活性增强。结论:DC细胞可增强CIK细胞的杀伤活性。To find better immunocompetent cells to cure leukemia. Methods：Lymphocytes were isola-ted from healthy volunteers＆#39; peripheral blood samples and separately cultured with cytokines at certain culture time to obtain DC and CIK cells after nine days. Harvested DC and CIK cells were co - cultured at a proportion of 1 to 5. Kill-ing effect of CIK cells on acute granulocyte leukemia cells of patients co - cultured with DC and CIK cells at different cellular proportion was detected. Results：The reproductive capacity and cytotoxic activity of CIK co - cultured with DC were significantly higher than those of CIK alone（P ﹤ 0. 05）. The cytotoxicity of CIK cells on acute granulocyte leukemia cells were increased with growing effector - target ratios. Conclusion：DC cells can enhance the cytotoxicity of CIK cells on acute granulocyte leukemia cells.

关 键 词：DC CIK 细胞 急性粒系白血病细胞 

分 类 号：R733.71[医药卫生—肿瘤]