CDC25B在肾癌细胞中的作用  

The effect of CDC25B in renal carcinoma cells

作　　者：于秀月[1] 孔垂泽[1] 张哲[1] 李振华[1] 

机构地区：[1]中国医科大学附属第一医院泌尿外科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2014年第8期1782-1785,共4页Journal of Modern Oncology

摘　　要：目的:探讨CDC25B在肾癌组织和细胞中的表达状况及对肾癌细胞凋亡、运动以及侵袭力的影响。方法:免疫组化检测肾癌组织中CDC25B的表达,Western blot和RT-PCR检测CDC25B蛋白在肾癌769-P和ACHN细胞中的表达。挑选表达量大的细胞系转染CDC25B shRNA。MTT法检测细胞增殖能力。Annexin V-FITC/PI-FCM实验检测肾癌细胞凋亡。Transwell检测肾癌细胞侵袭力。结果:CDC25B在肾癌组织中高表达。CDC25B在肾癌769-P和ACHN细胞中均表达,ACHN的恶性度高于769-P,CDC25B在ACHN中表达高于769-P。CDC25B蛋白被干扰后,随着时间的延长,肾癌ACHN细胞的增殖能力显著降低,各时间组与浓度组之间差异具有统计学意义。CDC25B蛋白被干扰后,肾癌ACHN细胞的凋亡增加。体外运动减弱。小室侵袭实验表明,干扰组的细胞数低于对照组,差异具有统计学意义(P<0.05)。干扰后降低了肿瘤细胞的侵袭能力。结论:CDC25B在肾癌中的表现为原癌基因的作用,CDC25B与肾癌的恶性程度呈正相关。CDC25B可能成为肾癌潜在的诊断标记和新的判定肿瘤恶性度的指标。To investigate the expression of CDC25B in renal cell carcinoma tissues and renal carcino-ma cells. To reveal the important role of CDC25B in renal carcinoma cell proliferation,apoptosis and invasiveness. Methods：Western blot and RT - PCR were used to detect the expression of CDC25B protein in renal cell carcinoma 769 - P and ACHN cells. We select the cell lines with the higher expression transfected with CDC25B shRNA. MTT was used to assay cell proliferation. Annexin V - FITC/ PI - FCM experiment was used to detect the apoptosis of renal cell carcinoma. Tanswell was used to detect renal cell carcinoma invasiveness. Results：CDC25B protein showed a high level of expression in tumor. CDC25B expressed both in renal cell carcinoma 769 - P and ACHN cells. The de-gree of malignancy of ACHN was higher than 769 - P. The expression of CDC25B in ACHN was higher than that in 769 - P. After the interference of CDC25B protein,with time lost,the cell proliferation of renal cell carcinoma ACHN was significantly decreased,a statistically significant difference was found between each time group and the concentra-tion group. The apoptosis of renal cell carcinoma ACHN was increased. It weakened renal cell carcinoma ACHN in vitro movement. Chamber invasion assay showed that the number of cells was less in interference group than the con-trol group（P ﹤ 0. 05）. Results showed that the interference weakens the invasive ability of tumor cells,compared with the control group. Conclusion：The role of CDC25B in renal cell carcinoma is as a proto - oncogene. The experiment indicated that CDC25B and malignant renal cell carcinoma were positively correlated. CDC25B may become a poten-tial renal cancer diagnostic marker and new indicator to determine the malignant degree.

关 键 词：CDC25B 肾细胞癌 ACHN 769-P 

分 类 号：R737.11[医药卫生—肿瘤]