乳腺癌患者外周血循环肿瘤细胞MiR-21检测的临床意义  

Clinical significance of plasma MiR- 21 detection circulating tumor cells as CTCs in breast cancer patients

作　　者：刘成兰[1] 马利 熊兵红[3] 程勇[3] 张才全[3] 

机构地区：[1]凉山州会东县人民医院检验科,四川凉山615200 [2]重庆市巴南区花溪(第二人民)医院内三科,重庆400054 [3]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《现代肿瘤医学》2014年第8期1853-1856,共4页Journal of Modern Oncology

摘　　要：目的:研究乳腺癌患者血浆中的MiR-21表达水平,初步探讨其在乳腺癌中作为循环肿瘤细胞(circulating tumor cells,CTCs)的临床意义。方法:采用基于SYBR Green I染料荧光分析的茎环实时定量逆转录聚合酶链式反应(qRT-PCR)分别检测40例乳腺癌患者及15例正常对照者外周血中MiR-21的表达水平,了解MiR-21表达与临床病理指标的关系。结果:乳腺癌患者血浆中的MiR-21表达水平明显高于正常对照者(P<0.01)。MiR-21表达与患者月经状况、肿瘤大小、雌激素受体(ER)、孕激素受体(PR)和人类表皮生长因子受体2(HER-2)表达均无明显关系(P>0.05),与TNM分期、淋巴结转移相关(P<0.05)。结论:茎环qRT-PCR是一种快速、准确、定量检测MiR-21的方法,MiR-21是一个能有效辅助乳腺癌诊断和监测CTCs的潜在标志物。To study the expression of MiR - 21 in peripheral blood in patients with breast cancer,and explore its clinical significance for diagnosing and monitoring GC,and serve as a marker for the detection of CTCs in breast cancer patients. Methods：Peripheral blood samples were collected from 40 preoperative patients with breast cancer and 15 healthy volunteers. Steploop real - time reverse transcription - polymerase chain reaction（qRT - PCR） was used to detect the level of MiR - 21. Results：Patients with breast cancer displayed a significantly higher level of MiR - 21 in peripheral blood than those from controls（P ﹤ 0. 01）. The MiR - 21 level was associated with the lymph node metastasis,TNM stage（P ﹤ 0. 05）,was not associated with menstrual station,size of tumor,the expression of ER,PR and HER - 2（P ﹥ 0. 05）. Conclusion：This study highlights the potential of the detection of MiR - 21 in pe-ripheral blood as a novel tool for diagnosis of GC and monitoring CTCs in breast cancer patients.

关 键 词：MIR-21 乳腺癌 qRT—PCR 血浆 循环肿瘤细胞 

分 类 号：R737.9[医药卫生—肿瘤]