骨髓间充质干细胞与肝星状细胞共培养后凋亡相关基因的改变  

Changes in apoptosis-related genes in bone marrow mesenchymal stem cells after cocultured with hepatic stellate cells

作　　者：胡昆鹏[1] 刘波[1] 姚志成[1] 林继宗[2] 邓美海[2] 潘卫东[2] 林楠[2] 陈骋[2] 许瑞云[2] 

机构地区：[1]中山大学附属第三医院岭南医院普通外科,广东省广州市510530 [2]中山大学附属第三医院肝胆外科,广东省广州市510630

出　　处：《中国组织工程研究》2014年第28期4444-4449,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家教育部博士点基金(20110171120089;20110171110070)~~

摘　　要：背景:前期研究证实骨髓间充质干细胞可在体外促进肝星状细胞凋亡、抑制其活性,但其作用机制尚未明确。目的:通过基因芯片技术,筛选出骨髓间充质干细胞调控肝星状细胞凋亡过程中可能参与的凋亡相关基因。方法:将分离提纯的人骨髓间质干细胞与肝星状细胞在6孔Transwell板上建立上下共培养体系,单独培养人骨髓间质干细胞作为对照组,培养72 h。基因芯片分析单独培养组与共培养组骨髓间充质干细胞凋亡相关基因的改变情况,找出与调控肝星状细胞密切相关的凋亡基因。结果与结论:采用SABiosciences第2代功能分类基因芯片产品进行凋亡基因筛查发现,共培养后骨髓间质干细胞中显著上调的凋亡基因有:AKT1,PIK3R2,DAPK1,DHCR24,NOTCH2,BDNF等。结合前期研究结果,推测NOTCH有可能在骨髓间充质干细胞调控肝星状细胞过程中起关键作用。BACKGROUND：Previous studies have confirmed that bone marrow mesenchymal stem cells in vitro can promote hepatic stel ate cellapoptosis and inhibit its activity, in which the mechanism of action remains unknown. OBJECTIVE：To screen out apoptosis-related genes during hepatic stel ate cellapoptosis regulated by bone marrow mesenchymal stem cells using gene chip technology. METHODS：Purified human bone marrow mesenchymal stem cells were seeded in 6-wel Transwel plate and cocultured with hepatic stel ate cells. Cultured human bone marrow mesenchymal stem cells alone served as control group, and cultured for 72 hours. The alterations in apoptosis-related genes were analyzed between culture alone group and coculture group using gene chip technology. The genes strongly associated with regulation of hepatic stel ate cells were selected. RESULTS AND CONCLUSION：By the functional classification of second-generation SABiosciences Gene chips, apoptotic gene screening found that after coculture, significantly upregulated genes in bone marrow mesenchymal stem cells contained：AKT1, PIK3R2, DAPK1, DHCR24, NOTCH2 and BDNF. Combined with previous findings, we hypothesized that NOTCH may play a key role in the regulation of hepatic stel ate cells by bone marrow mesenchymal stem cells.

关 键 词：干细胞 骨髓干细胞 肝星状细胞 骨髓间充质干细胞 凋亡 肝纤维化 肝硬化 基因芯片 

分 类 号：R394.2[医药卫生—医学遗传学]