肝癌干细胞分离及其细胞生物学特性  

Liver cancer stem cell isolation and biological characteristics

作　　者：刘岩[1] 周越塑[1] 赫兢[1] 刘鹏[1] 

机构地区：[1]解放军第三〇二医院急诊科,北京市100039

出　　处：《中国组织工程研究》2014年第28期4549-4554,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:正常干细胞和肿瘤干细胞在基因表达和依赖的细胞信号通路上应该存在不同,如何发现能选择性杀伤肿瘤干细胞的治疗手段是一个仍然需要大量研究的课题。目的:分离人肝癌细胞系肿瘤干细胞MHCC97,分析其细胞生物学特性。方法:采用流式细胞技术在人高转移肝癌细胞系MHCC97中筛选肿瘤干细胞,分离正常人肝脏干细胞CD133-CD34-MHCC97和人肝癌细胞系肿瘤干细胞CD133+CD34+MHCC97,分别检测其表型、生长曲线、细胞周期和多系分化能力。结果与结论:人肝癌细胞系CD133+CD34+MHCC97肿瘤干细胞的表型为CD133+CD34+,人肝癌细胞系CD133+CD34+MHCC97具有与CD133-CD34-MHCC97相似的细胞曲线和生长周期,可以向上皮和内皮细胞分化,并表达相应特异性的分子标志。提示人肝癌细胞系中CD133+CD34+MHCC97细胞具有肿瘤干细胞的特性,可以向内皮和上皮细胞分化,同时具有肿瘤干细胞的生物学特性,是肿瘤复发转移的根源,也是临床治疗的靶点。BACKGROUND：Normal stem cells and tumor stem cells have differences in cellsignaling pathway of gene expression and dependence. How to find a therapeutic method of selectively kil ing tumor stem cells is a topic that should be studied greatly. OBJECTIVE：To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them. METHODS：Tumor stem cells were selected from highly metastatic human hepatoma cellline MHCC97 by flow cytometry. CD133-CD34-MHCC97 from normal human liver stem cells and CD133＋CD34＋MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cellcycle and multi-lineage differentiation were measured. RESULTS AND CONCLUSION：Phenotypes of CD133＋CD34＋MHCC97 cancer stem cells were CD133＋and CD34＋and they had the same growth curve and cellcycle with the liver stem cells CD133-CD34-MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133＋CD34＋MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.

关 键 词：干细胞 肿瘤干细胞 人肝癌细胞系MHCC97 培养 分离 生物学特性 

分 类 号：R394.2[医药卫生—医学遗传学]