间充质干细胞移植修复炎性肠组织过程中的主导基因筛选  

Screening main genes during mesenchymal stem cell transplantation in repair of inflammatory bowel tissue

作　　者：曹燕雯[1,2] 魏亚明[3] 李瑜元[1,2] 聂玉强[1,2] 吴倩[1,2] 

机构地区：[1]广州医学院附属广州市第一人民医院消化科 [2]广州市临床医学研究所,广东省广州市510180 [3]广州医学院附属广州市第一人民医院输血科,广东省广州市510180

出　　处：《中国组织工程研究》2014年第28期4555-4562,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81070385);留学回国人员科研启动基因(教外司留{2012}940号);广州市重大民生专项基金(201300000100)~~

摘　　要：背景:前期研究已证明间充质干细胞可定植于炎症性肠黏膜修复炎症性肠组织。目的:应用表达谱芯片技术研究间充质干细胞移植修复前后大鼠炎症性大肠组织的差异基因表达情况,初步揭示与间充质干细胞定植、分化、修复炎症性肠组织过程相关的主导基因。方法:健康SD大鼠随机分为2组:移植实验组用三硝基苯磺酸灌肠制作炎症性肠病模型,造模后24 h经尾静脉注入绿色荧光蛋白标记的间充质干细胞;对照组以生理盐水代替三硝基苯磺酸同法灌肠处理。于移植后28 d取移植实验组和对照组大肠组织,利用基因芯片技术筛选移植实验组和对照组的差异表达基因。结果与结论:对照组和移植实验组大肠组织全基因组表达谱芯片结果有差异性表达的基因共388个(P<0.05,FC>2),其中表达上调基因191个、表达下调基因197个,主要与炎症反应、免疫反应和细胞分化3个方面有关。前10位显著上调和下调的差异基因(共20条)中,与炎症反应相关的有3个,与免疫反应相关的有3个,与干细胞分化相关的有2个。388个差异基因主要涉及33条信号通路(P<0.05),其中与炎症反应相关的有6条,与免疫反应相关的有8条,与干细胞分化相关的有5条。结果可见利用全基因组表达谱基因芯片的方法初步筛选了间充质干细胞修复炎症性肠组织的主要相关基因。BACKGROUND：Previous studies have verified that mesenchymal stem cells could be transplanted into inflammatory bowel mucosa to repair inflammatory bowel tissue. OBJECTIVE：To observe the differential gene expression in large intestine before and after mesenchymal stem celltransplantation in repair of inflammatory bowel tissue of rats using microarray technology, and to primarily discover the main genes during mesenchymal stem celltransplantation, differentiation, and reparation in inflammatory colorectal tissue region. METHODS：Healthy Sprague-Dawley rats were randomly divided into two groups. Experimental rat models of inflammatory bowel disease were established using trinitrobenzene sulfonic acid via enema. At 24 hours after model establishment, green fluorescent protein-labeled mesenchymal stem cells were infused via the caudal vein. The control group was treated with physiological saline by enema, instead of trinitrobenzene sulfonic acid. At 28 days, large intestine was obtained from the experimental group and control group. Differential y expressed genes were screened in the experimental and control groups using microarray technique. RESULTS AND CONCLUSION：The microarray analysis results showed that there were 388 differential genes in the control and experimental groups （P〈0.05, FC〉2）, in which 191 were up-expressed, and 197 were down-expressed. Al of these genes were mainly involved in inflammatory reaction, immune reaction and celldifferentiation. In the top 10 up-regulation and down-regulation differential genes （total y 20 genes）, 3 genes were involved in inflammation, 3 genes were involved in immune reaction, and 2 genes were related to stem celldifferentiation. In the 388 genes, 33 were related to signaling pathways （P〈0.05）, 6 related to inflammation, 8 related to immunity, and 5 related to stem celldifferentiation. Results suggested that the main genes involved in mesenchymal stem cells in repair of inflammatory bowel tissue were primarily screened using gene expression microa

关 键 词：干细胞 移植 炎症性肠病 间充质干细胞 表达谱基因芯片 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]