口腔鳞状细胞癌组织中差异微小RNA/mRNA表达谱的对接研究  

Differential expression profiles of microRNAs/mRNA and docking study in oral squamous cell carcinoma

作　　者：刘思玉[1,2] 谢龙[1,2] 祁兵[3] 马长艳[4] 桑磊[1,2] 李宏卫[1,2] 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院口腔颌面外科 [3]南京医科大学病理科 [4]南京医科大学细胞生物学与医学遗传学系,南京210029

出　　处：《华西口腔医学杂志》2014年第4期400-403,共4页West China Journal of Stomatology

基　　金：江苏高校优势学科建设工程基金资助项目(2014-37)

摘　　要：目的构建分析口腔鳞状细胞癌(OSCC)中差异表达的微小RNA(miRNA)和mRNA表达谱,初步预测与OSCC发生和发展相关的miRNA和mRNA。方法运用高通量深度测序技术构建miRNA和mRNA表达谱,通过Gene Ontology功能显著性富集分析预测与OSCC细胞周期、增殖、分化和凋亡相关的miRNA和mRNA。结果共发现77个miRNA和1 298个mRNA显著性差异表达,富集分析显示与OSCC细胞周期、增殖、分化和凋亡相关的miRNA共73个,且一个miRNA可调控多个mRNA。结论 OSCC中差异表达的miRNA和mRNA对OSCC的发生和发展有潜在的作用。Objective To construct and analyze the differential expression profiles of microRNAs （miRNA） and mRNA in oral squamous cell carcinoma （OSCC）. To explore the miRNA and mRNA of OSCC development and progression. Methods The differential expression profiles of miRNA and mRNA were built by high-throughput deep sequencing technology. Using Gene Ontology （GO） enrichment analysis, the roles of differentially expressed miRNA and mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis were analyzed. Results Seventy-seven differentially expressed miRNA and 1 298 differentially expressed mRNAs were identified in OSCC. GO analysis showed that 73 miRNA had found target mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis of OSCC. Moreover, a miRNA could regulate multiple mRNA. Conclusion The differential expression profiles of miRNA and mRNA have close relationship with the development and progression of OSCC.

关 键 词：口腔鳞状细胞癌 微小RNA MRNA 高通量测序 

分 类 号：R739.8[医药卫生—肿瘤]