Snail、E-cadherin和N-cadherin在非小细胞肺癌组织的表达及相关性研究  

The Expressions of Snail,E-Cadherin and N-Cadherin in Non-small Cell Lung Cancer and the Correlation Analysis

作　　者：吴安邦 覃可乐 朱广[2] 汤轶[2] 肖高明[1,2] 陈跃军[1,2] 

机构地区：[1]南华大学,湖南衡阳421001 [2]中南大学湘雅医学院附属肿瘤医院胸外科,湖南长沙410013

出　　处：《肿瘤药学》2014年第4期253-257,共5页Anti-Tumor Pharmacy

基　　金：湖南省科技厅基金项目资助(2009FT3151)

摘　　要：目的探讨上皮型钙黏附素(E-cadherin)、神经型钙黏附素(N-cadherin)及Snail在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法采用实时荧光定量PCR测定Snail、E-cadherin和N-cadherin基因在10对非小细胞肺癌及其癌旁正常肺组织中的表达情况,同时采用免疫组化法检测105例NSCLC以及41例癌旁组织中Snail、E-cadherin和N-cadherin蛋白的表达。结果与癌旁正常肺组织相比较,非小细胞肺癌组织中E-cadherin的表达显著减少,N-cadherin和Snail表达明显增加(P<0.05)。NSCLC组织中,Snail的表达与N-cadherin的表达呈明显的正相关(P<0.05,r=0.21),与E-cadherin的表达呈显著负相关(P<0.05,r=-0.39),而N-cadherinl的表达与E-cadherin的表达也呈明显负相关(P<0.05,r=-0.53)。结论非小细胞肺癌组织中,Snail、N-cadherin呈显著高表达,而E-cadherin呈显著低表达,三者可能通过相互作用共同参与NSCLC的发生和发展。Objective To investigate the expressions of snail, E-cadherin and N-cadherin in non-small cell lung can-cer （NSCLC） and their clinical significance. Methods The quantitative real-time reverse transcriptase polymerase chain reaction （qRT-PCR） was used to detect the Snail, E-cadherin and N-cadherin mRNA levels in 10 cases of NSCLC tis-sues and the tissues adjacent to carcinoma. Then the immunohistochemistry （IHC） was applied to detect the protein levels of Snail, E-cadherin and N-cadherin in 105 cases of non-small cell lung cancer and 41benign pulmonary tissues. Results Compared with the benign pulmonary tissues, the mRNA and protein expression of E-cadherin in NSCLC tissues were significantly decreased, while the mRNA and protein expression of Snail and N-cadherin were both obviously increased （P〈0.05）. In NSCLC tissues, the Snail expression was positively correlated with N-cadherin expression （P〈0.05, r=0.21） and negatively correlated with E-cadherin expression （P〈0.05, r=-0.39）, and the N-cadherin expression was also nega-tively correlated with E-cadherin expression （P〈0.05, r=-0.53）. Conclusion In NSCLC tissues, the expressions of Snail and N-cadherin were both up-regulated, while the E-cadherin expressions were significantly down-regulated. All of them may be involved in the occurrence and development of NSCLC through their interaction.

关 键 词：SNAIL 上皮型钙黏附素 神经型钙黏附素 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]