rhuPAa-melittin的生物活性检测  

Detection of biological activity of rhuPAa-melittin

作　　者：李城 金世光 李昌熙 王大新 颜炜群 

机构地区：[1]扬州大学临床医学院疼痛科,江苏扬州225001

出　　处：《实用临床医药杂志》2014年第16期11-13,39,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省十二五"科教兴卫工程"创新团队与领军人才(LJ201159);江苏省科技支撑计划-社会发展项目(BE02010697);973重点项目(2007CB936104)

摘　　要：目的利用高纯度的rhuPAa-melittin进行人成纤维细胞和卵巢癌细胞进行活性检测,探讨rhuPAa-melittin对卵巢癌治疗作用及对正常细胞毒性作用。方法利用高纯度的rhuPAa-melittin蛋白分别作用于人成纤维细胞和人卵巢癌细胞SKOV3 48 h,在490 nm波长处测定其吸光度,以反映活细胞的数量并间接反映rhuPAa-melittin对培养中细胞的生长抑制作用。结果人卵巢癌细胞SKOV3组,在4μg/mL时对细胞抑制率已经达到了66.59%,在16μg/mL时,细胞已经全部死亡,差异有统计学意义(P<0.05)。而人成纤维细胞组,在4μg/mL时其对细胞的抑制率仅为达到7.3%,8μg/mL时抑制率为12.3%,16μg/mL时抑制率为22.0%。结论 rhuPAa-melittin对人卵巢癌细胞SKOV3细胞有着明显的抑制杀伤作用,而对于人成纤维细胞的抑制作用不明显。Objective RhuPAa-melittin protein with high-purity was analyzed to investigate its biologic effect on normal and ovarian cancer cells. Methods Preliminary purified rhuPAa-melittin was added in medium normal cells and SKOV3 human ovarian cancer cells cultured for 48 hours,and then were measured at 490 nm wavelength absorbance to measure the number of living cells and evaluate the inhibitory effect of rhuPAa-melittin on cultured cells. Results The inhibitory rate of rhuPAamelittin at 4 μg /mL was 66. 59% in SKOV3 cancer cell progression. At a concentration of 16 μg /mL,rhuPAa-melittin killed all cancer cells（P〈0. 05）. In normal adult fibrous cells,the inhibitory rate of rhuPAa-melittin at 4 μg /mL was only 7. 3%,and 12. 3% inhibition rate was obtained by using rhuPAa-melittin（8 μg /mL）. Moreover,inhibition rate topped at 22. 0% when used rhuPAa-melittin at16 μg /mL. Conclusion RhuPAa-melittin has a strong anti-tumor effect on SKOV3 cancer cells.

关 键 词：rhuPAa-melittin 成纤维细胞 卵巢癌细胞 

分 类 号：R737.31[医药卫生—肿瘤]