ET-1及CXCR4蛋白在人脑胶质瘤中的表达及其意义  

Expression and significance of ET-1 and CXCR4 protein in human brain glioma

作　　者：何毅[1] 纪涛[1] 陶晶[1] 

机构地区：[1]广东省深圳市第二人民医院神经外科,广东深圳518000

出　　处：《实用临床医药杂志》2014年第16期50-52,共3页Journal of Clinical Medicine in Practice

基　　金：广东省深圳市科技计划项目编号(201102061)

摘　　要：目的探讨人脑胶质瘤组织中ET-1及CXCR4蛋白的表达及其与胶质瘤病理级别的关系。方法采用SP免疫组化技术检测人脑胶质瘤和正常脑组织中的表达,并用彩色图像分析系统做定量分析。结果在人脑胶质瘤和正常脑组织中,ET-1及CXCR4蛋白均可表达;其高表达与人脑胶质瘤预后不良相关,且在正常脑组织中的表达水平显著低于人脑胶质瘤（P〈0.01）;Ⅰ-Ⅱ级和Ⅲ-Ⅳ级人脑胶质瘤组织中ET-1和CXCR4蛋白表达均高于正常脑组织,差异有统计学意义（P〈0.01）,并且ET-1及CXCR4蛋白在人脑胶质瘤Ⅲ~Ⅳ级组中的表达高于Ⅰ-Ⅱ级组,差异有统计学意义（P〈0.01）。结论人脑胶质瘤和正常脑组织中均有ET-1及CXCR4蛋白的表达,且在人脑胶质瘤中呈显著高表达;ET-1及CXCR4的表达可能与人脑胶质瘤的发生、发展以及人脑胶质瘤的病理分级及预后相关。Objective To explore the expression and significance of ET-1 and CXCR4 protein in human brain glioma. Methods Immunohistochemical technique was used to detect the expressions of ET-1 and CXCR4 protein in human brain glioma and normal cerebral tissues,and the result was analyzed by the image system. Results The expressions of ET-1 and CXCR4 protein in glioma tissues of different malignant grades were significantly different. Higher expressions of ET-1 and CXCR4 were associated with poor prognosis. The expression levels of ET-1 and CXCR4 protein in glioma of gradeⅠ ~ Ⅱ and Ⅲ ~ Ⅳ were higher than those in normal cerebral tissues（P〈0. 01）,and the ET-1 and CXCR4 protein level in glioma of grade Ⅲ- Ⅳ was higher than those in glioma of gradⅠ -Ⅱ. Conclusion ET-1 and CXCR4 protein have been detected in human glioma and normal cerebral tissues,and the expression of ET-1 and CXCR4 protein in human glioma is higher,which might be closely related to the occurrence and development of glioma. The staining intensity of the positive nuclei of ET-1 and CXCR4 can evaluate the prognosis and reflect the malignant grade of glioma.

关 键 词：ET-1 CXCR4 胶质瘤 SP免疫组化 预后 

分 类 号：R739.41[医药卫生—肿瘤]