慢病毒介导的α-烯醇化酶基因沉默对宫颈癌细胞化疗药物敏感性的影响  

Effects of Lentivirus Mediated Alpha Enolase Gene Silencing on Drug Sensitivity of Cervical Cancer Cells

作　　者：张栋栋[1,2] 刘会玲[3] 范维[1] 王玉凤[4] 陈小芸[1] 郭钰珍[1] 

机构地区：[1]兰州大学第二医院,兰州730000 [2]桓台县人民医院,淄博256400 [3]甘肃省人民医院,兰州730000 [4]兰州大学第一医院,兰州730000

出　　处：《世界科技研究与发展》2014年第4期398-401,共4页World Sci-Tech R&D

基　　金：甘肃省科技计划(1105TCYA016)资助

摘　　要：目的探讨沉默α-烯醇化酶(ENO1)基因对宫颈癌SiHa细胞化疗药物敏感性的影响。方法采用慢病毒介导的RNA干扰技术,建立ENO1基因稳定沉默SiHa细胞系;通过western blot方法验证ENO1的表达水平;ENO1基因沉默后运用细胞增殖实验检测其对细胞增殖能力的影响;采用MTT的方法评价沉默ENO1基因的SiHa细胞对紫杉醇和顺铂的敏感性。结果成功建立ENO1基因沉默的SiHa细胞系;沉默ENO1基因表达后,SiHa细胞增殖能力下降;沉默ENO1基因能够增强SiHa细胞的药物敏感性。结论:ENO1能够影响宫颈癌细胞的增殖及其对化疗药物的敏感性。Objective To evaluate the effects of gene silencing of α-enolas（ENO1）on cervical cancer SiHa cells.Methods A lentiviral system harboring both enhanced the ENO1 short hairpin RNA expression cassette was constructed.Then the sta-ble ENO1 gene silencing SiHa cell lines were established.Western blot analysis of proteins were used to evaluate the expres-sion of ENO1.The chemosensitivity of paclitaxel and cisplatin of cervical cancer with α-enolas gene silencing cell were iden-tified by MTT.Results The successful construction of ENO1gene lentiviral vector can silence in SiHa cells.The gene silen-cing of α-enolas can inhibit proliferation and increas drug sensitivity of cervical cancer cells.Conclusion ENO1 can affect the proliferation and sensitivity to chemotherapeutic drugs of cervical cancer cells.

关 键 词：ENO1 基因沉默 紫杉醇 顺铂 药物敏感性 

分 类 号：R737.33[医药卫生—肿瘤]