UCF -101对大鼠脑缺血再灌注损伤p38 MAPK 通路的影响  

Effect of UCF -101 on the p38 MAPK signal pathway during focal cerebral ischemia -reperfusion in rats

作　　者：苏丹颖[1] 马晶[2] 孙亮[2] 张丽[1] 王永贵[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,黑龙江哈尔滨150001 [2]哈尔滨医科大学解剖教研室,黑龙江哈尔滨150081

出　　处：《中国急救医学》2014年第8期739-742,I0002,共5页Chinese Journal of Critical Care Medicine

基　　金：黑龙江省科技厅科学技术攻关项目（GC12C305-2）

摘　　要：目的：探讨UCF-101在大鼠脑缺血再灌注损伤中的作用及分子机制。方法采用线栓法建立Wistar大鼠大脑中动脉闭塞（ middle cerebral arteryocclusion ， MCAO）模型，随机分为假手术组、缺血再灌注组和UCF-101组，术后观察神经功能缺损症状，TUNEL法检测神经元凋亡，免疫组化法及 Western blot 检测脑皮层神经元磷酸化 p38丝裂原活化蛋白激酶（ mitogen activated protein kinase ， MAPK）蛋白的表达。结果 UCF-101改善大鼠脑缺血再灌注后的神经功能缺损症状，减少细胞凋亡（P＜0．05）；与假手术组比较，大鼠缺血后磷酸化p38 MAPK表达水平增加；与脑缺血组比较，UCF-101组磷酸化p38 MAPK表达水平有所降低（ P＜0．05）。结论UCF-101对脑缺血再灌注损伤有保护作用，其机制可能与p38 MAPK信号通路有关。Objective To investigate the neuroprotective effect and mechanisms of 5 -[5-（2-nitrophenyl）furfuryliodine]-1,3-diphenyl-2-thiobarbituric acid （UCF-101） on the cerebral neurons of rats during cerebral ischemia -reperfusion.Methods The focal cerebral ischemia models of Wistar rats were established by the middle cerebral artery occlusion （ MCAO ） with thread occlusion methods .Rats were randomly divided into sham operated group , ischemia -reperfusion group and UCF-101 treated group .24 h after focal cerebral ischemia -reperfusion , the rats were evaluated for neurological deficits and TUNEL method was used to measure apoptotic neurons , and the expression levels of p38 MAPK phosphorylation were detected by immunohistochemistry and Western blot analysis . Results UCF -101 treatment significantly improved neurological behavior and reduced TUNEL -positive cells in the cerebral cortex . Furthermore , the phosphorylation levels of p 38 MAPK were increased in ischemia-reperfusion group thanthose in sham operated group （P〈0.05）.In UCF-101 treated group the phosphorylation levels of p 38 MAPK was decreased compared with the ischemia -reperfusion group （ P 〈0 .05 ） . Conclusion p38 MAPK signal pathway might be involved in neuroprotective effect of UCF -101 treatment on focal ischemic brain of mice .

关 键 词：UCF-101 脑缺血再灌注 脑保护 磷酸化水平 UCF-101 

分 类 号：R364.12[医药卫生—病理学]