富血小板血浆/藻酸盐缓释载体复合物修复骨缺损的实验研究  

Study of repairing the bone defect with platelet-rich plasma/alginate sustained release composites

作　　者：李思敏[1] 郭良微[1] 周晋[2] 高云飞[1] 

机构地区：[1]哈尔滨医科大学附属口腔医院牙周病科 [2]哈尔滨医科大学附属第一医院血液科,哈尔滨150001

出　　处：《国际口腔医学杂志》2014年第5期546-551,共6页International Journal of Stomatology

基　　金：黑龙江省博士后基金(LBH-Z11065);黑龙江省自然科学基金(D200831)

摘　　要：目的比较富血小板血浆(PRP)/藻酸盐微囊和PRP/藻酸盐微珠2种复合材料的生物学性能。方法 42只新西兰兔分为3组,每组14只,切除兔桡骨中下段1.5 cm的骨质造成极限性骨缺损模型,分别植入PRP/藻酸盐微珠复合物、PRP/藻酸盐微囊复合物、PRP凝胶于骨缺损处。另取4只桡骨同样部位和大小骨缺损旷置作为空白对照。术后观察其一般情况并于8、12周每组分别处死7只实验动物,取材行X线片检查及组织学切片,观察成骨效果。结果术后8周和12周各组骨缺损区均有新骨形成,成骨量随时间的推移而增加。经影像学检查和组织学评估,各组的成骨能力依次为:PRP/藻酸盐微珠>PRP/藻酸盐微囊>PRP/凝胶,各组间的差异有统计学意义(P<0.05),其中PRP/藻酸盐微珠的成骨能力最强。结论 PRP/藻酸盐微珠对骨缺损愈合有明显促进作用,可以作为组织工程学中一种替代骨组织的生物活性材料。Objective This study aimed to investigate and compare the biological potential of two types of plateletrich plasma（PRP） loaded alginate hydrogel（PRP alginate beads and PRP alginate capsules） through animal tests in vivo. Methods A critical size defect of 15mm elongation was created in the mid-upper part of bilateral radius of 45 New Zealand white rabbits and either suppied with PRP alginate beads（14 rabbits）, and PRP alginate capsules（14 rabbits）, PRP gel（14 rabbits）, and untreated（4 rabbits）. After 8, 12 weeks of implantation, the gross, radiological and histological observations were performed to investigate the bone healing of the defect. Results All of the defects treated with transplant materials exhibited new bone formation 8 and 12 weeks postoperatively, increasing with time.The radiological and histomorphological evaluation showed that the ability of new bone formation was arranged in 3 groups as follows： PRP alginate beads〉PRP alginate capsules〉PRP gel, the difference was significant between them（P〈0.05）. Successful bone regeneration was observed at the bone defect applied（treated） with PRP alginate beads, which is marked contrast to other groups. Conclusion PRP alginate beads could be a promising material capable of enhancing bone regeneration.

关 键 词：富血小板血浆 藻酸盐 缓释载体 桡骨缺损 

分 类 号：R781.42[医药卫生—口腔医学]