芦笋多糖对肿瘤小鼠红细胞离子通道活性的影响  

Effect of Asparagus Polysaccharide on Erythrocyte Ion Channel in Tumor Model Mice

作　　者：季宇彬[1,2] 汲晨锋[1,2] 

机构地区：[1]国家教育部抗肿瘤天然药物工程研究中心哈尔滨商业大学,哈尔滨150076 [2]生命科学与环境科学研究中心哈尔滨商业大学,哈尔滨150076

出　　处：《中国食品学报》2014年第7期27-31,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(30400592);黑龙江省自然科学基金项目(C200612)

摘　　要：目的:研究芦笋多糖对肿瘤小鼠红细胞离子通道活性的影响。方法:建立了肿瘤动物模型,分高、中、低剂量腹腔给予芦笋多糖7 d,采集并制备红细胞悬液,用试剂盒结合分光光度计测定肿瘤小鼠红细胞膜Na+,K+-ATPase、Ca2+,Mg2+-ATPase活性,采用Fluo-3/AM、MQAE、BCECF-AM荧光探针染色,使用激光共聚焦显微镜和荧光分光光度计分别测定小鼠红细胞内[Ca2+]、[CI-]、[H+]浓度的变化。结果:芦笋多糖能升高肿瘤小鼠红细胞膜Na+,K+-ATPase、Ca2+,Mg2+-ATPase活性,降低肿瘤小鼠红细胞内[Ca2+]浓度,增强红细胞阳离子通道转运活性。芦笋多糖能够降低肿瘤小鼠红细胞内[CI-]浓度,增强红细胞阴离子通道转运活性。芦笋多糖还能够降低肿瘤小鼠红细胞内的[H+]浓度,升高红细胞内pH,维持红细胞酸碱平衡。结论:芦笋多糖通过提高肿瘤小鼠红细胞膜离子通道的功能,稳定细胞内环境,从而有助于保护红细胞膜结构,维持红细胞发挥正常功能。Objective： To study the effect of Asparagus polysaccharide on erythrocyte ion channel in tumor model mice. Methods： Tumor mice model was made and treated with different dosage of Asparagus polysaccharide for 7 days,and suspensions of erythrocytes were separated from anticoaguted whole blood, spectrophotometer with test kit was used to measure Na＋,K＋-ATPase, Ca2＋, Mg2＋-ATPase activity, erythrocytes were dyed with Fluo-3/AM, MQAE, BCECF-AM and laser scanning confocal microscope and fluorescence spectrophotometry were used to determine [Ca2＋], [CI-] and [H＋]. Results： Asparagus polysaccharide could increase erythrocyte Na＋, K＋-ATPase, Ca2＋,Mg2＋-ATPase activity, lower [Ca2＋] concentration, improve cation channel transport activity. Asparagus polysaccharide could lower [CI-] concentration, improve anion channel transport activity. Asparagus polysaccharide could increase intra-cellular pH, maintain acid-base balance.Conclusion： Asparagus polysaccharide can improve erythrocyte ion channel transport activity, maintain cell inner environment and protect cell structure, enhance erythrocyte immune function.

关 键 词：芦笋 多糖 肿瘤小鼠 红细胞 离子通道 

分 类 号：TQ281[化学工程—有机化工] R285.5[医药卫生—中药学]