高浓度氧气吸入对ARDS大鼠肺组织氧化应激的影响  

The impact of inhalation high concentration of oxygen on oxidative stress in the lung tissue of the ARDS rats

作　　者：刘玉琪[1] 谭国良[1] 孙旭日[1] 何鲤穗[1] 陈志量[1] 

机构地区：[1]福建医科大学附属第二医院重症医学科,福建泉州326000

出　　处：《中国现代医学杂志》2014年第19期15-20,共6页China Journal of Modern Medicine

基　　金：福建医科大学校苗圃基金(No:2010MP001);福建省泉州市优秀人才基金(No:10A15);福建省泉州市科技立项(No:2010Z35)

摘　　要：目的探讨ARDS大鼠吸入高浓度氧气后肺组织氧化应激的变化。方法实验动物分为正常大鼠组和ARDS大鼠组,每组30只,每组再以吸入空气、60%氧气、100%氧气各分成三个亚组,每个亚组10只。72 h后留取动脉血行血氧分压(PO2)及丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单胺氧化酶(MAO)测定;肺组织行HE染色观察Smith评分,RT-PCR检测Ⅱ型肺泡上皮细胞(AECⅡ)特异性肺泡表面活性蛋白C(SPC)和Ⅰ型肺泡上皮细胞(AECⅠ)特异性水通道蛋白5(AQP5)的mRNA表达。结果各组大鼠吸氧72 h后均存活。与正常大鼠空气亚组比较,正常大鼠100%氧气亚组Smith评分升高(3.77±1.20比0.83±0.26),P<0.05;MDA升高(2.39±0.92比0.82±0.31),P<0.05;SOD下降(51.99±21.73比86.44±26.38),P<0.05;AQP5 mRNA下降(0.67±0.25比0.91±0.28),P<0.05;ARDS大鼠空气亚组的Smith评分升高(11.65±3.82比0.83±0.26),P<0.05;MDA升高(5.73±2.43比0.82±0.31),P<0.05;SOD下降(45.71±14.68比86.44±26.38),P<0.05;CAT下降(0.52±0.23比0.85±0.34),P<0.05;AQP5 mRNA下降(0.28±0.12比0.91±0.28),P<0.05;SPC mRNA下降(0.33±0.14比0.72±0.28),P<0.05。ARDS组大鼠空气亚组的Smith评分(11.65±3.82比3.77±1.20),P<0.05和AQP5 mRNA(0.28±0.12比0.67±0.25),P<0.05;与正常大鼠100%氧气亚组差异显著。MAO在各组内和组间均差异无显著性(P>0.05)。ARDS组大鼠动脉血氧分压100%氧气亚组高于60%氧气亚组(112.86±26.32比86.94±21.07),P<0.05,60%氧气亚组高于空气亚组(86.94±21.07比65.05±15.19),P<0.05。在ARDS组内与空气亚组比较,60%氧气亚组(0.43±0.18 VS 0.28±0.12),P<0.05和100%氧气亚组(0.47±0.23比0.28±0.12),P<0.05的AQP5 mRNA均有显著性升高;100%氧气亚组的Smith评分降低(7.88±2.53比11.65±3.82),P<0.05,SPC mRNA升高(0.58±0.25比0.33±0.14),P<0.05。结论高浓度氧气吸入和ARDS均可以影响氧自由基,氧自由基并不是高浓度氧的特征性产物,难以作为客观有效的氧毒性监测指标;高浓度氧气吸入对�[Objective]To investigate the impact of inhalation high concentrations of oxygen on oxidative stress in the lung tissue of the ARDS rats. 【Methods】30 healthy adult rats were used for establishing the ARDS model by injecting LPS, and the other 30 rats wereusedas normal group. Both of the rats in two groups were divided into three subgroups respectively by inhaling different concentrations of oxygen（air, 60% oxygen and 100% oxygen）. After 72hours the rats were killedand the partial pressure of oxygen（PO2）, malondialdehyde（MDA）, superoxide dismutase（SOD）, Catalase（CAT） and monoamine oxidase（MAO） were measured in the arterial blood, the smith score was calculated by HE staining from the lung tissue,the ultrastructure was observed by transmission electron microscopy,the expression of SPC mRNA and AQP5 mRNA were detected by RT-PCR of lung tissue. 【Results】All of the rats in each group survived after inhalation oxygen for 72 hours. Compared with the air subgroup of normal rats, the Smith score（3.77±1.20 VS 0.83±0.26, P 0.05） and MDA（2.39±0.92 VS 0.82±0.31, P 0.05） were increased,whileSOD（51.99±21.73 VS 86.44±26.38, P 0.05） and AQP5 mRNA（0.67±0.25 VS 0.91±0.28, P 0.05） was decreased in the 100% O2 subgroup of normal rats; in the air subgroup of ARDS rats, the Smith score（11.65±3.82 VS 0.83±0.26, P 0.05） and the MDA（5.73±2.43 VS 0.82±0.31, P 0.05） were higher, while the SOD（45.71±14.68 VS86.44±26.38, P 0.05） and CAT（0.52±0.23 VS 0.85±0.34, P 0.05） were lower, the expression of AQP5 mRNA（0.28±0.12 VS 0.91±0.28, P 0.05） and SPC mRNA（0.33±0.14 VS 0.72±0.28, P 0.05） were lower too. The difference of the Smith score（11.65±3.82 VS 3.77±1.20, P 0.05） and the expression of AQP5 mRNA（0.28±0.12 VS0.67±0.25, P 0.05） was significantly between the air subgroup of ARDS rats and the 100% O2 subgroup of normal rats. There were no difference of MAO between two groups and in each subgroup. In the ARDS group, the PO2of100% O2 subgroup was

关 键 词：高浓度氧气 ARDS 氧自由基 AQP5 SPC 

分 类 号：R-332[医药卫生]