高糖环境下锰超氧化物歧化酶转染间充质干细胞促进抗氧化酶类表达  

Manganese superoxide dismutase transfers the mesenchymal stem cells to promote the expression of antioxidant enzyme in the high glucose conditions

作　　者：张鹏[1] 刘琰[2] 李萌[1] 纪亮[1] 沈雷[1] 王玉[3] 谢婷婷[4] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院第三附属医院呼吸科,黑龙江齐齐哈尔161006 [3]齐齐哈尔医学院基础医学研究所,黑龙江齐齐哈尔161006 [4]齐齐哈尔医学院

出　　处：《中国现代医学杂志》2014年第20期27-30,共4页China Journal of Modern Medicine

基　　金：黑龙江省教育厅指令科研课题(No:12521635)

摘　　要：目的研究锰超氧化物歧化酶(MnSOD)转染间充质干细胞(MSC)发挥抗自由基的细胞保护作用,并分析其机制。方法利用pcDNA3.1-MnSOD质粒转染MSC作为MnSOD-MSC实验组,仅转染pcDNA3.1为空白质粒组,未转染者为MSC对照组,在正常或高糖(含250 mmoL葡萄糖)培养条件下,MTT法观察MSC增殖情况,使用试剂盒检测各组MSC中谷胱甘肽氧化还原酶(GSHPX)、超氧化物歧化酶(SOD)表达情况。结果高糖环境下,与MSC对照组相比,MnSOD-MSC实验组MSC增殖OD值增高(P<0.01);与空白质粒组GSHPX相比,MnSOD-MSC实验组GSHPX含量明显增高(P<0.01),MnSOD-MSC实验组SOD含量在高糖条件下比空白质粒组增高(P<0.01)。结论 MnSOD转染MSC可以促进抗氧化酶类表达增高,对抗自由基,对MSC具有保护作用。[Objectives] To research cell protective effects against free radicals of mesenchymal stem cells （MSC） transfeeted by manganese superoxide dismutase （MnSOD）, and to analyze its mechanism. [Metb- otis] pcDNA3.1-MnSOD plasmid transfecting MSC was used as MnSOD-MSC experimental group, peDNA3.1 transfecting only as blank plasmidgroup, untransfected as the MSC control group. Under normal or high sugar （including 250 mmol glucose） cultivating conditions, MTF method was used to observe the MSC prolifera- tion, and expression of glutathione oxidoreductase （GSHPX） and superoxide dismutase （SOD） in MSC of each group was detected by kits. [Results] Under high sugar environment, compared with MSC control group, in the MnSOD-MSC experimental group, OD value of MSC proliferation increased, with P 〈0.01; compared with the blank plasmid group GSHPX, in the MnSOD-MSC experimental group, GSHPX content increased obviously, with P〈0.01; in the MnSOD-MSC experimental group, under high sugar environment, content of SOD was higher than the content of SOD in the blank plasmid group, with P〈0.01. [Conclusion] MnSOD transfecting MSC can promote increase of antioxidant enzyme expression, with the function of fighting free radicals and protecting MSC.

关 键 词：锰超氧化物歧化酶 间充质干细胞 谷胱甘肽还原氧化酶 超氧化物歧化酶 

分 类 号：R641[医药卫生—外科学] Q813.11[医药卫生—临床医学]