人参茎叶霜冻前后的质量变化  

Research on quality changes in ginseng stems and leaves before and after frost

作　　者：赵岩[1] 马爽[1] 蔡恩博[1] 刘双利[1] 杨鹤[1] 张连学[1] 王士杰[2] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林农业科技学院中药学院,吉林吉林132101

出　　处：《中国中药杂志》2014年第16期3117-3122,共6页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BAI03B01);国家公益性行业科研专项(201303111);吉林省科技发展计划项目(20126046;20140204013YY);吉林省中医药产业发展专项(YYZX201258);国家自然科学基金项目(31000154)

摘　　要：采用HPLC测定人参茎叶受冻前后单体皂苷、游离氨基酸含量,紫外分光光度法测定总酚含量及DPPH法测定自由基清除能力的变化,考察人参茎叶霜冻前后的质量变化。结果表明,受冻后人参茎中9种单体皂苷含量除Rg1,Re升高外,其余皂苷含量均有所下降,人参叶中皂苷含量均明显降低;受冻后人参茎中游离氨基总含量降低,而人参叶氨基酸总含量升高;受冻后人参茎、叶中的总酚含量降低,但对自由基的清除能力提高。霜冻对人参茎叶质量产生一定的影响。The present study is to investigate the quality changes of ginseng stems and leaves before and after frost. The contents changes of ginsenoside,free amino acid,and total phenolic compounds,as well as DPPH radical scavenging effect before and after frost were measured. The content of 9 ginsenoside monomer in ginseng stems was decreased except for Rg1 and Re after frost,but in ginseng leaves was all decreased. The total content of amino acids was decreased in ginseng stems after frost,while increased in ginseng leaves. The content of phenolic compounds in ginseng stems and leaves were both decreased after frost while the ability of DPPH radical scavenging was improved. The factor of frost has great impact on the quality of ginseng stems and leaves.

关 键 词：人参茎叶 霜冻 人参皂苷 氨基酸 DPPH清除率 

分 类 号：R284.2[医药卫生—中药学] O657.72[医药卫生—中医学]