微晶酚酞分散固相萃取-分光光度法测定痕量亚硝酸根  

Determination of Trace Nitrite by Dispersive Solid Phase Extraction Based on Microcrystalline Phenolphthalein

作　　者：丁宗庆[1] 李小玲[2] 

机构地区：[1]郧阳师范高等专科学校生物化学与环境工程系,湖北十堰442000 [2]湖北医药学院附属人民医院临床医学研究所,湖北十堰442000

出　　处：《分析科学学报》2014年第4期566-568,共3页Journal of Analytical Science

基　　金：湖北省教育厅科学技术研究项目(No.Q2009601);郧阳师范高等专科学校重点科研项目(No.2012A01)

摘　　要：研究了微晶酚酞固相萃取-分光光度法测定痕量亚硝酸根。高浓度的酚酞乙醇溶液滴入水中后,酚酞会析出大量微晶,对疏水性物质具有很强的吸附萃取能力,据此建立了痕量亚硝酸根测定的新方法。详细考察了影响萃取富集效率的多种因素,在最佳实验条件下,该方法的线性范围为1.0～160μg/L（r=0.9999）,检出限为0.29μg/L。方法已成功应用于实际水样分析,加标回收率在98.1%～103.6%之间,相对标准偏差为1.9%～3.4%。A novel method using dispersive solid phase extraction based on microcrystalline phenolphthalein by spectrophotometry was established for the determination of trace nitrite. When a high concentration of phenolphthalein in ethanol solution is dripped into water, phenolphthalein will precipitate and form plenty of microcrystalline, which has a strong ability of sorptive extraction to hydrophobic material. Accordingly,we built a new method for the measurement of trace nitrite. A various of factors that influence the efficiency of extraction preconcentration were examined in detail. Under the optimum conditions,the linear range of the method was 1.0～160μg/L（r=0.9999） and the detection limit was 0.29μg/L. The proposed method,with the recovery ranging in 98. 1%-103.6% and relative standard deviations（RSDs） ranging in 1.9 %-3.4 %, has been successfully applied to the analysis of water samples.

关 键 词：微晶酚酞 分散固相萃取 亚硝酸根 分光光度法 

分 类 号：O657.32[理学—分析化学]