白细胞介素-8对CD133＋肝癌干细胞侵袭转移能力的影响  

Effect of interleukin-8 on migration and invasion of CD133 ＋ positive liver cancer stem cells

作　　者：张翔[1] 林承杰[1] 黄新辉[1] 曾永毅[1] 刘景丰[1] 

机构地区：[1]福建医科大学附属第一医院肝胆胰外科二区,福州350005

出　　处：《中华实验外科杂志》2014年第8期1706-1708,共3页Chinese Journal of Experimental Surgery

基　　金：福建省自然科学基金资助项目（2011J01162）;福建省卫生厅青年课题（2012-2-47）;福建省属高校基金资助项目（JK2011021）

摘　　要：目的 观察白细胞介素（IL）-8对CD133＋肝癌干细胞侵袭转移能力的影响并探讨其机制.方法 磁珠分选MHCC97-H细胞,检测上清液中IL-8表达,比较CD133＋/CD133-细胞克隆形成、裸鼠成瘤、迁移侵袭能力.IL-8中和抗体处理细胞并分组,比较克隆形成、迁移侵袭能力.结果 CD133＋细胞表达更高水平的IL-8[（85.76±2.55） ng/L比（13.40 ±2.19） ng/L],细胞浓度为1×106/L时即可成瘤;其克隆形成[（13.50±0.62）％比（4.43±0.31）％]、迁移[（39.3±3.2）个比（18.0±2.6）个]、侵袭[（37.5±3.0）个比（20.3±1.8）个]能力强于CD133-细胞.IL-8中和抗体处理后,CD133＋/CD133-细胞的克隆形成率降低[（4.63±0.47）％比（12.93±0.55）％,（3.53±0.49）％比（6.67±0.15）％],CD133＋细胞的迁移[（24.7±2.8）个比（37.7±2.8）个]、侵袭[（25.2±2.1）个比（40.2±3.6）个]能力降低,CD133-细胞无明显变化.结论 IL-8可特异性地参与调节CD133＋肝癌干细胞的侵袭转移能力.Objective To study the effect and possible mechanism of interleukin （IL）-8 on migration and invasion of CD133 ＋ liver cancer stem cells （LCSCs）.Methods CD133 ＋ cells were isolated by using magnetic-activated cell sorting （MACS） from MHCC97-H cells.Migration,invasion and colony formation efficiency of CD133 ＋/CD133-cells were detected.IL-8 in the supernatants was detected by enzyme linked immunosorbent assay （ELISA）.Tumor-forming ability was observed by xenografts in the BALB/c nude mice.The effect of IL-8 neutalizing antibody on migration,invasion and colony formation efficiency of the sorted cells was determined.Results IL-8 was up-regulated in CD133 ＋ cells [（85.76 ± 2.55） ng/L] as compared with CD133-cells [（13.40 ± 2.19） ng/L].The CD133 ＋ cells exhibited a greater ability of colony formation efficiency [（13.50 ± 0.62） ％ vs.（4.43 ± 0.31） ％],migration [（39.3 ± 3.2） vs.（18.0 ± 2.6）],invasion [（37.5 ± 3.0） vs.（20.3 ± 1.8）] and tumors initiation.Colony formation efficiency was decreased in both CD133 ＋ [（4.63 ± 0.47）％ vs.（12.93 ± 0.55）％] and CD133-[（3.53 ±0.49）％ vs.（6.67 ±0.15）％] cells treated by IL-8 neutralizing antibody.IL-8 neutralizing antibody suppressed the migration [（24.7 ± 2.8） vs.（37.7 ± 2.8）] and invasion [（25.2 ± 2.1） vs.（40.2 ± 3.6）] of the CD133 ＋ cells specifically.Conclusion CD133 plays a functional part in regulating migration and invasion of LCSCs via regulating IL-8 signaling.

关 键 词：白细胞介素-8 CD133 肝癌干细胞 侵袭 转移 

分 类 号：R735.7[医药卫生—肿瘤]