载脂蛋白ApoE修饰脂质体对肝癌细胞的靶向转染  

Target transfer of liposome combined with apolipoprotein E to hepatoma carcinoma cells

作　　者：秦琴[1] 莱智勇 高然朋[3] 牛凯[4] 于保锋[3] 解军[3] 徐钧[5] 

机构地区：[1]山西省人民医院中心实验室,太原030012 [2]山西医科大学 [3]山西医科大学生物化学与分子生物学教研室 [4]山西省人民医院急诊科,太原030012 [5]山西省人民医院普外科,太原030012

出　　处：《中华实验外科杂志》2014年第8期1709-1711,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81172136、30901821）;山西省高等学校创新人才支持计划资助项目

摘　　要：目的 将载脂蛋白E （ApoE）和普通脂质体（Lipoplexes）耦联,构建一种具有肝癌细胞靶向性的基因转运载体ApoE-Lipoplexes,以提高转染效率.方法 将ApoE与Lipoplexes耦联,形成ApoE-Lipoplexes,分别用两种载体包裹pGenesil-1质粒并转染HepG2和Huh-7肝癌细胞,用倒置荧光显微镜观察增强型绿色荧光蛋白（EGFP）的表达,并用流式细胞仪检测转染效率,比较两种载体对肝癌细胞的转染效率.结果 构建了粒径150 nm的Lipoplexes和ApoE-Lipoplexes两种脂质体,Lipoplexes对HepG2和Huh-7的转染效率分别为（4.7±1.4）％和（21.0±0.6）％,ApoE-Lipoplexes的转染效率分别为（13.8±1.8）％和（51.5±1.8）％,ApoE-Lipoplexes对肝癌细胞的转染效率显著高于Lipoplexes（P＜0.05）.结论 成功构建一种对肝癌细胞具有较高转染效率的非病毒基因转运载体.Objective To construct a new delivery vector which is combined apolipoprotein E （ApoE） with liposome to taget hepatoma carcinoma cells.Methods ApoE was combined with liposome （Lipoplexes） to form a new delivery vetor （ApoE-Lipoplexes）,and the plasmid pGenesil-1 with enhanced green fluorescent protein （EGFP） tag encapsulated by both vectors was transferred into HepG2 and Huh-7 cells,respectively.The expression of EGFP was observed under inverted fluorescent microscope,and the transfer efficiency was measured by flow cytometry and compared between the two vectors.Results The diameters of Lipoplexes and ApoE-Lipoplexes were 150 nm.The transfer efficiency of Lipoplexes to HepG2 and Huh-7 was （4.7 ± 1.4） ％ and （21.0 ± 0.6） ％,and that of ApoE-Lipoplexes was （13.8 ± 1.8） ％ and （51.5 ± 1.8）％,respectively.The transfer efficiency of ApoE-Lipoplexes on hepatoma carcinoma cells was significantly higher than that of Lipoplexes （P ＜ 0.05）.Conclusion This new nonviral liposome vector with ApoE has a higher transfer efficiency for hepatoma carcinoma cells.

关 键 词：基因治疗 载脂蛋白E 癌 肝细胞 靶向治疗 

分 类 号：R735.7[医药卫生—肿瘤]