川桑丝氨酸蛋白酶抑制剂基因的鉴定与表达模式分析  

Identification and Expression Pattern Analysis of the Serine Protease Inhibitor Genes inMorus notabilis

作　　者：王裕鹏[1] 梁九波[1] 丁光宇 李文生[1] 杨光伟[1] 何宁佳[1] 

机构地区：[1]家蚕基因组生物学国家重点实验室西南大学蚕学与系统生物学研究所,重庆400716

出　　处：《蚕业科学》2014年第4期582-591,共10页ACTA SERICOLOGICA SINICA

基　　金：中央高校基本科研业务费专项(No.2362014xk05);国家自然科学基金青年基金项目(No.31300228)

摘　　要：丝氨酸蛋白酶抑制剂(SPI)主要参与蛋白酶活性的调节,植物SPI具有抵御病原菌侵染和昆虫取食危害的功能。通过比对MEROPS数据库以及保守氨基酸基序的分析,在川桑(Morus notabilis)基因组中鉴定到34个SPI蛋白。这些SPI蛋白可分为Kunitz、Serpin和PI-Ⅰ3个家族,依据氨基酸序列中的半胱氨酸残基数目又将Kunitz家族成员分为4类。氨基酸多序列比对表明Serpin和PI-Ⅰ家族成员在序列上相对保守。基于川桑根、皮、花、芽、叶的RNAseq数据量化的RPKM值分析发现不同类型SPI基因的组织表达模式存在差异,暗示川桑不同类型SPI行使不同的生物学功能。以广东桑(Morus atropurpurea)品种粤桑-69851的幼叶作为创伤处理材料,检测PI-Ⅰ家族的6个SPI基因对创伤诱导响应的表达模式,其中有4个基因不同程度地受到创伤诱导而上调表达,推测它们在桑树防御昆虫咬食危害中具有重要作用。Serine protease inhibitors（SPIs） are primarily involved in proteolytic regulation,which confer plants with defense ability against microorganism infection and insect attack.Through sequence comparison in MEROPS database and analysis of conserved amino acid motifs,34 SPIs were found to be encoded by the Morus notabilis genome.These SPIs could be classified into three families,namely Kunitz,Serpin and PI-Ⅰ.The Kunitz family SPIs could be further divided into four groups based on the number of cysteine residue.Multiple sequence alignment showed that Serpin and PI-Ⅰfamily SPIs had more conserved sequences.In addition,based on the RPKM data of RNAseq,different gene expression patterns were observed in three families of SPI genes in the M.notabilis root,bark,flower,bud,and leaf,suggesting that different SPIs play diverse biological roles in Morus notabilis.The mulberry cultivar of M.atropurpurea Yuesang-69851 was used to detect the wound-induced expression patterns of 6 PI-Ⅰ family genes.The results showed that expressions of 4 PI-Ⅰ family genes were more or less upregulated by wounding treatment,suggesting their potential roles in the defense against insect attack.

关 键 词：川桑 丝氨酸蛋白酶抑制剂 基因家族 表达模式 

分 类 号：S888.2[农业科学—特种经济动物饲养] Q786[农业科学—畜牧兽医]