脱胶溶液pH值对蚕丝脱胶效果和丝素蛋白分子质量的影响  

Effect of Degumming Solution pH Value on Degumming of Silk Fiber and Molecular Weight of Silk Fibroin

作　　者：张小平[1] 梅锦秾 吕佳[1] 王宇阳[1] 卢神州[1] 

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215123

出　　处：《蚕业科学》2014年第4期699-705,共7页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.51373114);江苏省自然科学基金项目(No.BK20131176);南通市科技计划项目(No.BK2012095)

摘　　要：丝素蛋白的分子质量会直接影响到丝素材料的性能。以弱碱盐NaHCO3和Na2CO3为脱胶剂,配制NaHCO3-Na2CO3缓冲溶液调节pH值进行蚕丝脱胶试验,通过检测蚕丝脱胶率、染色K/S值、丝素蛋白溶液黏度,观察脱胶蚕丝的表面形貌以及凝胶电泳测定丝素蛋白分子质量等,分析确定脱胶溶液pH值对蚕丝脱胶效果和丝素蛋白的分子质量都有较大影响。随脱胶溶液pH值增加,蚕丝的脱胶率逐渐增大,但pH值达到10.5以后,蚕丝表面丝素会受到较为严重的腐蚀;随脱胶溶液pH值增加,丝素蛋白的分子质量逐渐减小,丝素蛋白溶液的黏度逐渐降低。调节蚕丝脱胶溶液的pH值对于保持丝素蛋白的分子质量及保证丝胶的完全脱除很重要。Molecular weight of silk fibroin affects the performance of fibroin material directly.In this study,NaHCO3-Na2CO3 buffer solutions with different pH values were prepared as degumming agents to conduct the degumming of silk fiber.Their effects on degumming rate,K/S value of dye,viscosity of silk fibroin aqueous solution,surface feature of degummed fibroin,and molecular weight of silk fibroin were examined.The results showed that pH value of degumming solution had remarkable effect on the degumming of silk fiber and molecular weight of silk fibroin.Silk degumming rate increased with pH value.However,when the pH value was over 10.5,the surface of silk fibroin was corroded severely.Moreover,the molecular weight of silk fibroin and the viscosity of fibroin aqueous solution were reduced gradually.It is important to choose an appropriate pH value to keep the molecular weight of silk fibroin and to ensure complete removal of sericin.

关 键 词：蚕丝 脱胶溶液 pH值 脱胶率 丝素蛋白 分子质量 

分 类 号：TS143.32[轻工技术与工程—纺织材料与纺织品设计]