响应面法优化海洋镰刀腐皮菌产出HMG-CoA还原酶抑制剂的培养条件  

Use of response surface methodology on the production of active compound MFS from Fusarium solani and its activity on HMG-CoA reductase

作　　者：周喻[1] 吴文惠[2,3] 苏同伟[1] 包斌[1,2] 王小雨[1] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工与贮藏工程技术研究中心,上海201306 [3]上海海洋大学海洋科学研究院,上海201306

出　　处：《微生物学通报》2014年第8期1516-1524,共9页Microbiology China

基　　金：国家863计划项目(No.2011AA09070109)

摘　　要：【目的】采用响应面法对海洋微生物镰刀腐皮菌FG319发酵产生MFS(Metabolite of Fusarium solani FG319)培养条件进行优化。【方法】在单因素试验结果的基础上,依据Box-Behnken中心组合原则设计诱导物添加量、培养时间、培养温度的3因素3水平响应面实验,以MFS产出量为响应值优化镰刀腐皮菌FG319的培养条件。【结果】镰刀腐皮菌FG319最优培养条件为诱导物添加量0.6%、培养时间7 d、培养温度22°C,在此培养条件下,MFS最高产量达到20.11 mg/L,是优化前的4倍,与理论预测的相对误差为0.64%,实测值与响应面预测值拟合良好。镰刀腐皮菌FG319代谢产物MFS在HMG-CoA还原酶和NADPH构筑的分子评价反应体系,当MFS添加到100 mg/L时,具有类似洛伐他汀抑制HMG-CoA还原酶的最大抑制率。【结论】响应面试验设计对镰刀腐皮菌FG319培养条件的优化是有效的,其次生代谢产物MFS体外抑制HMG-CoA还原酶的效果也是明显的。[Objective] In the present study, response surface methodology （RSM） was used to optimize the fermentation conditions of MFS （metabolite of Fusarium solani FG319） extracted from marine microorganism, Fusarium solani. [Methods] On the basis of single factor tests, Box-Behnken RSM was employed with three factors such as addition of L-ornithine hydrochloride at different concentrations, culture time, and culture temperature on the yield of MFS. [Results] The optimum culture conditions were as follows： the L-omithine hydrochloride addition was 0.6% （W/V）, culture time was 7 d, culture temperature was 22 ~C. Under these conditions the yield of MFS was 20.11 mg/L, which is 4 times increased than without any optimization and the relative error with the theoretical value was 0.64%. The inhibition rate of MFS on HMG-CoA reductase was achieved above 100 mg/L, and has a similar inhibition as Lovastatin. ]Conclusion] The culture conditions obtained by response surface methodology were effective and reliable, and the effect of MFS on HMG-CoA reductase is obvious in vitro.

关 键 词：响应面法 培养条件 海洋微生物 HMG-COA还原酶 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]