肝硬化大鼠细菌易位以及甜菜碱的干预作用  

Bacterial translocation in rats with hepatic cirrhosis and the interventional effect of betaine

作　　者：陈云霞[1] 张慧英[2] 孟莉[1] 李旭炯[3] 来丽娜[4] 田小霞[2] 王黎敏[5] 

机构地区：[1]长治医学院微生物学教研室,山西长治046000 [2]长治医学院病理生理学教研室,山西长治046000 [3]长治医学院生理学教研室,山西长治046000 [4]长治医学院药理学教研室,山西长治046000 [5]长治医学院机能综合实验室,山西长治046000

出　　处：《微生物学通报》2014年第8期1629-1636,共8页Microbiology China

基　　金：国家自然科学基金项目(No.81070339);山西省国际科技合作计划项目(No.2010081068);山西省回国留学人员科研基金项目(No.211-091);山西医科大学细胞生理学省部共建教育部重点实验室主任基金项目(No.2010-09);长治医学院博士科研启动经费项目(No.2010-01)

摘　　要：【目的】研究肝硬化大鼠的细菌易位情况,并探讨甜菜碱对其的干预作用。【方法】随机将48只健康雄性SD(Sprague dawley,SD)大鼠分为四组:正常对照组(N),甜菜碱干预的正常对照组(NB),肝硬化模型组(M),甜菜碱干预的肝硬化模型组(MB)。采用复合致病因素法诱导大鼠肝硬化,NB组和MB组使用1 000 mg/(kg w·d)的甜菜碱水溶液灌胃,N组和M组使用等体积饮用水灌胃;HE染色观察肝脏与小肠损伤情况,并检测各组动物脏器指数与细菌易位情况。【结果】M组大鼠体重增长缓慢(与N组比较,4周和6周时间点均P=0,P<0.01);与M组相比,MB组大鼠体重增长较快,至6周时间点体重差异显著(P=0.023,P<0.05)。与N组相比,M组动物4周时间点肝脏指数显著升高(P=0,P<0.01);6周时间点肝脏(P=0,P<0.01)、脾脏指数(P=0.038,P<0.05)均显著升高,肾脏指数显著降低(P=0.019,P<0.05);与M组相比,6周时间点MB组动物肝脏指数(P=0.038,P<0.05)明显降低,肾脏指数(P=0.011,P<0.05)明显升高。M组动物肝、肠组织发生明显病理学改变;MB组动物病理学改变减轻。M组发生细菌易位的大鼠数量升高,4周时主要易位于MLN,6周时主要易位于MLN和肾脏;MB组发生细菌易位的大鼠数量有所减少,其中6周时易位至MLN的大鼠数量明显减少(P=0.046,P<0.05)。【结论】复合致病因素诱导的肝硬化大鼠,发生细菌易位的器官主要是MLN和肾脏,易位的细菌可通过淋巴管道转位,并随肝硬化病程进展趋于严重。甜菜碱除了其转甲基的保护作用以外,很可能还通过对肠道的保护作用,在一定程度上阻止了肝硬化动物细菌易位的发生。[Objective] To study bacterial translocation of rats with hepatic cirrhosis, and to explore the interventional effect of betaine on bacterial translocation. [Methods] Forty-eight male SD rats were randomly divided into four groups, two groups were normal control group （N group and NB group）, and the other two were hepatic cirrhosis models （M group and MB group）. In each condition, one group of rats was intragastrieally administered with 1 000 mg/kg body weight betaine once daily for 4 weeks and 6 weeks （NB or MB group）, whereas the other was treated with an equal volume water （N or M group）. The rat model of hepatic cirrhosis was induced by multiple pathogenic factors. The injury of liver and small intestine was observed with HE stain. The organ indices were detected, and the bacterial translocation was assessed by standard microbiological techniques on blood, mesenteric lymph nodes, ascites, liver, spleen and kidney. [Results] The weight of M group was found to be significantly lower compared to N group （P=0, P〈0.01）, and the weight of MB group was picked up compared to M group, it was significantly higher at the end of 6 weeks （P=-0.023, P〈0.05）. In M group to N group, the liver index （P=0, P〈0.01） was significantly higher at the end of 4 weeks; the organ index of liver （P=0, P〈0.01） and spleen （P=0.038, P〈0.05） was significantly higher, and that of kidney （P=0.019, P〈0.05） was significantly lower at the end of 6 weeks. And the organ index of liver （P=0.038, P〈0.05） was fell back, that of kidney （P--0.011, P〈0.05） was picked up in MB group to M group. The pathologic change of liver and small intestine was obvious in M group to N group, and it was reduced in MB group to M group. The bacterial translocation in M group was higher than that in N group, and it occurred mainly in MLN at the end of 4 weeks, in MLN and kidney at the end of 6 weeks; the bacterial translocation was lower in MB group than that in M group, especially in mesenteric lymph nodes

关 键 词：大鼠 肝硬化 细菌易位 甜菜碱 干预作用 

分 类 号：R575.2[医药卫生—消化系统]