泰山枯草芽孢杆菌的分离鉴定及其对黄曲霉毒素B_1的降解研究  

Study on Isolation and Identification of Mountain Tai Bacillus subtilis and its Degradation of Aflatoxin B_1

作　　者：孙玲玉[1] 李超[1] 郝海玉[1] 柴同杰[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国畜牧兽医》2014年第8期246-250,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2012BAD39B0205);国家环境保护环境微生物利用与安全控制重点实验室开放基金资助(MARC2011D061)

摘　　要：本试验旨在从泰山土壤中筛选出具有降解黄曲霉毒素B1(AFB1)作用的枯草芽孢杆菌,经16SrRNA基因测序鉴定为枯草芽孢杆菌,最终确定1株具有较高降解作用的枯草芽孢杆菌,命名为泰山枯草芽孢杆菌,并利用该菌株对AFB1进行了降解率、活性组分、性质的确定及降解饲料中AFB1效果的研究。结果表明,泰山枯草芽孢杆菌对AFB1的降解率达90.28%,其中,泰山枯草芽孢杆菌发酵后的上清液对AFB1的降解活性最高,确定发酵后的上清液中存在解毒活性组分;对解毒活性物质进行加热和蛋白酶K变性处理后,解毒活性显著降低,表明起解毒作用的活性物质是其分泌的一种胞外酶;应用性试验进一步证明泰山枯草芽孢杆菌能降解霉变饲料中的AFB1,可在饲料或食品行业应用。The aim of this experiment was to select the Bacillus subtilis which was useful for the degradation of aflatoxin B1 from the soil of Mountain Tai.Through the 16 SrRNA gene sequencing,we identified one Bacillus subtilis which had high degradation effect of aflatoxin B1,named as Mountain Tai Bacillus subtilis,the determination of degradation efficiency and active components of aflatoxin B1 and the effect of degradation aflatoxin in feed were researched in this experiment used Mountain Tai Bacillus subtilis.The results showed that the degradation rate of aflatoxin B1 of Mountain Tai Bacillus subtilis was 90.28%,the degrading efficiencies of aflatoxin B1 by exocellular coarse extraction liquid was highest,the activity of aflatoxins degradation mainly happened in the culture supernatant of Mountain Tai Bacillus subtilis.Characteristic research of active component including heating and degeneration of proteinase K,the detoxification decreased significantly,which suggested that the effect of supernatant from Mountain Tai Bacillus subtilis on aflatoxin B1 might be due to the enzymatic reactions.Further application tests proved that Mountain Tai Bacillus subtilis could degrade aflatoxin B1 in mouldy feed and it could be applied in the feed or food industry.

关 键 词：枯草芽孢杆菌 分离鉴定 AFB1 发酵上清液 降解酶 

分 类 号：S852.6[农业科学—基础兽医学]