大孔树脂-硅胶柱层析法纯化花生根中白藜芦醇  

Study on Purification of Resveratrol from Peanut Root by Macroporous Resin and Silica Gel Column Chromatography

作　　者：陈琼玲[1] 刘红芝[1] 刘丽[1] 何轩辉[1] 王强[1] 胡晖[1] 

机构地区：[1]中国农业科学院农产品加工研究所农业部农产品加工综合性重点实验室北京100193

出　　处：《中国食品学报》2014年第6期127-132,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：2009公益性行业科研专项(200903043);国家科技支撑计划项目(2012BAD29B00)

摘　　要：研究了大孔树脂-硅胶柱层析法分离纯化花生根中白藜芦醇的工艺。通过动静态解吸附试验确立大孔树脂最佳分离工艺为:大孔树脂为聚酰胺,上样质量浓度26.58μg/mL,解吸剂为80%乙醇,洗脱流速2 BV/h。通过薄层色谱与硅胶柱层析试验,确定最佳纯化条件为:洗脱剂V(氯仿)∶V(甲醇)=40∶1、流速30 mL/min、m(硅胶)∶m(样品)=1∶1。产物经V(甲醇)∶V(氯仿)=3∶1重结晶后,采用高效液相色谱测定其纯度为97.01%。The technology of purification resveratrol from peanut roots by macroporous resin and medium-pressure silica gel column chromatography was investigated. The optimum separation conditions, studied by static and dynamic adsorption and desorption experiments, were as follows： macroporous resin polyamide, sample concentration 26.58 μg/mL, eluent 80% ethanol, desorption flow rate 2 BV/h. Based on the thin-layer chromatography and silica gel column chromatography experiments, it was found that the crude extracts could be purified on the conditions of eluent chloroform/ methanol=40：1（V/V）, flow rate 30 mL/min, silica gel/sample=1：1（m/m）. After recrystallization by methanol/chloroform=3： 1, the purity of resveratrol was 97.01% determined by HPLC.

关 键 词：白藜芦醇 大孔树脂 硅胶柱层析 分离纯化 

分 类 号：TQ914.1[化学工程]