四种水体微生物总DNA提取方法的比较  

Comparison of Several Methods for Extraction of Total Community for Water

作　　者：赖树锦 郭佳[1] 陶新园 李阳[1] 杨振飞[1] 陈鑫[1] 李晓华[1] 

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室,武汉430074

出　　处：《湖北农业科学》2014年第10期2440-2442,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31070087;30570046);湖北省自然科学基金重点项目(2011CDA079);国家大学生创新创业训练项目(GCX13104);湖北省烟草公司面上资助项目(0710XYYC2012-01)

摘　　要：用Roling法、SDS法、CTAB改进法和Crump改进法提取水体微生物总DNA,并以细菌的16S rDNA通用引物进行PCR扩增。研究结果表明,四种提取方法提取的水体微生物总DNA大小都在23 kb以上,Roling法提取的水体微生物总DNA的浓度明显小于其他3种方法,Crump改进法提取的水体微生物总DNA的A260 nm/A280 nm和A260 nm/A230 nm比值高于其他3种方法,Crump改进法所提取的微生物总DNA可以直接用于后续的PCR扩增试验。The total community DNA from water was extracted by the Roling method, SDS method, CTAB method and Crump method respectively, then the universal primers for 16S rDNA was used for PCR amplification.The results showed that the DNA extractions were above 23.1 kb. The lesser amount of DNA and little impurities were extracted by Roling method , Highest amount of DNA was extracted by Crump method . Which A 260/A 280 andA 260/A 230 ratio is higher than other three meth-ods.

关 键 词：水体微生物 总DNA提取 DNA质量 PCR扩增 

分 类 号：Q939[生物学—微生物学]