白色念珠菌白斑中核苷酸结合寡聚化结构域1、核因子κB及人β-防御素的表达  

Expression of nucleotide-binding oligomerization domain1, nuclear factor-kappa B and human beta-defensins in candidal albicans leukoplakia

作　　者：钱雅洁[1,2] 蒋文晖[3] 王翔[1,2] 段宁[1,2] 黄晓峰[3] 王文梅[1,2] 

机构地区：[1]南京大学医学院附属口腔医院,210008 [2]南京大学口腔医学院口腔黏膜病科,210008 [3]南京大学口腔医学院病理科,210008

出　　处：《中华口腔医学杂志》2014年第8期464-469,共6页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81070839）;江苏省创新团队与领军人才项目（LJ201110）

摘　　要：目的 研究白色念珠菌白斑和无白色念珠菌感染的白斑患者组织中核苷酸结合寡聚化结构域1 （nucleotide-binding oligomerization domain 1,NOD1）、核因子κB及人β-防御素1,2,3的表达,探讨白色念珠菌感染对口腔白斑组织中NOD1信号通路内关键蛋白和人β-防御素表达的影响.方法 收集40例口腔黏膜白斑组织样本,采用HE染色、过碘酸-雪夫（periodic acid-Schiff,PAS）染色、真菌银染法和免疫组化法检测,四项均阳性者19例,判定为白色念珠菌白斑,其余21例判定为无白色念珠菌感染白斑.采用蛋白质印迹法检测白色念珠菌白斑和无白色念珠菌感染的白斑组织中NOD1和核因子κB的表达水平并进行比较.采用免疫组化法检测白色念珠菌白斑和无白色念珠菌感染的白斑组织中NOD1、核因子κB和人β-防御素1,2,3的表达,图像分析测定平均吸光度值,对平均吸光度值行独立样本t检验,检验水准为双侧α=0.05.结果 在40例口腔白斑中,白色念珠菌阳性率为48％（19/40）.蛋白质印迹法结果显示白色念珠菌白斑中NOD1、核因子κB蛋白表达水平较无白色念珠菌感染的白斑显著降低.免疫组化结果表明,无白色念珠菌感染的白斑中NOD1、核因子κB和人β-防御素1,2,3染色的吸光度值分别为0.31 ±0.02、0.47±0.03、0.42±0.02、0.53±0.04、0.47±0.03,白色念珠菌白斑中NOD1、核因子κB和人β-防御素1,2,3染色的吸光度值分别为0.25±0.01、0.30±0.02、0.35±0.02、0.42±0.03、0.36±0.02,白色念珠菌白斑组织中NOD1、核因子κB和人β-防御素1,2,3的表达水平均较无白色念珠菌感染组显著减弱,差异均有统计学意义（P＜0.05）.结论 白色念珠菌感染可能通过抑制NOD1信号通路降低口腔白斑组织中人β-防御素的表达.Objective To examine the expression of nucleotide-binding oligomerization domain 1 （NOD1）,nuclear factor-kappa B（NF-κB） and human beta-defensins in candidal albicans leukoplakia and to investigate the effect of candida albicans infection on key proteins in NOD 1 signaling pathway and the expression of human beta-defensin.Methods Forty cases of oral leukoplakia samples were collected and stained by hematoxylin-eosin staining,periodic acid-Schiff staining,silver staining and immunohistochemical methods.Nineteen samples were positive with these four methods and judged as candidal albicans leukoplakia,and the other twenty-one samples judged as leukoplakia without candidal albicans infection.Western blotting was used to detect the expressions of NOD1 and NF-κB in these forty samples.In addition,the immunohistochemical method was adopted to investigate the relationship between NOD 1,NF-κB,human beta-defensin 1,2,3 expressions and candida albicans.Results The positive rate of candida albicans in oral leukoplakia was 48%（19/40）.The expressions of NOD1 and NF-κB in the candida albicans leukoplakia were lower than that in leukoplakia without candida albicans infection.The mean optical density value of NOD 1,NF-κB,human beta-defensin 1,2,3 in candidal albicans leukoplakia were 0.25±0.01,0.30±0.02,0.35±0.02,0.42±0.03,0.36±0.02 respectively,which were significantly lower than that in leukoplakia without candida albicans infection （0.31±0.02,0.47±0.03,0.42±0.02,0.53±0.04,0.47±0.03） （P〈0.05）.Conclusions By inhibiting the NOD1 signaling pathway,candida albicans infection may reduce the expression level of human beta-defensin 1,2,3 in oral leukoplakia.

关 键 词：念珠菌 白色 Β-防御素 白斑 口腔 核苷酸结合寡聚化结构域1 

分 类 号：R781.5[医药卫生—口腔医学]