靶向livin的siRNA对鼻咽癌细胞增殖和凋亡的影响  

Effects of siRNA targeting livin gene on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z

作　　者：何跃平[1] 罗晶晶[2] 刘钊[1] 安立[1] 李祥东[1] 张艳[3] 

机构地区：[1]遵义医学院第五附属珠海医院耳鼻喉科,珠海519100 [2]南华大学附属第一医院麻醉科,衡阳421001 [3]南华大学病原生物学研究所,衡阳421001

出　　处：《中国免疫学杂志》2014年第8期1028-1031,共4页Chinese Journal of Immunology

基　　金：湖南省卫生厅科研项目(C2005044)资助

摘　　要：目的:观察livin基因siRNA对鼻咽癌细胞CNE-2Z增殖和凋亡的影响。方法:将靶向livin基因的siRNA真核表达质粒pGPU6/GFP/Neo-livin通过脂质体介导转染至鼻咽癌细胞系CNE-2Z,半定量RT-PCR、Western blot分别检测livin基因mRNA和蛋白表达,caspase-3活性检测试剂盒检测caspase-3的活性,MTT法检测细胞增殖活性,流式细胞仪检测细胞凋亡率。结果:与未转染(空白对照)和阴性对照组(pGPU6/GPF/Neo-shNC,无关序列对照组)CNE-2Z细胞比较,实验组(pGPU6/GPF/Neo-livin)重组质粒使livin基因mRNA和蛋白表达明显下降,表达抑制率分别为64.38%和61.43%;caspase-3活性增强,细胞增殖活力受到抑制,细胞凋亡率增加。结论:靶向livin的siRNA抑制CNE-2Z细胞增殖并促进其凋亡。To observe the effect of livin gene-specific siRNA interference on proliferation and apoptosis of nasopharyngeal carcinoma （NPC） cell line CNE-2Z.Methods： siRNA expression vectors pGPU6/GFP/Neo-livin were transfected into NPC cell line CNE-2Z by using Lipofectamine 2000.The expressions of livin mRNA and protein were detected by semi-quantitative reverse transcriptase-polymerase chain reaction （ RT-PCR） and Western blot.The changes of caspase-3 activity were assessed by kinase semi-quantitative activity test.The proliferation activity and apoptosis of CNE-2Z cells were examined by MTT and flow cytometry respectively.Results：The expression levels of livin mRNA and protein in pGPU 6/GPF/Neo-livin transfected CNE-2Z cells were significantly lower than those in untreated and pGPU 6/GFP/Neo-shNC （ control non-target siRNA ） transfected cells with the expression inhibitory rate of 64.38% and 61.43% respectively.The caspase-3 activity and the apoptotic rate of experimental group cells were increased obviously.The growth of CNE-2Z cells was inhibited by siRNA recombinant expression vector transfection.Conclusion：siRNA targeting livin gene inhibits proliferation and induce apoptosis of CNE-2Z cells.

关 键 词：RNA干扰 LIVIN 鼻咽癌细胞 细胞凋亡 

分 类 号：R739.6[医药卫生—肿瘤]