Cyr61对人腺样囊性癌细胞系生物学特性的影响  

The effects of Cyr61 on the biological behavior of human adenoid cystic cancer cells

作　　者：张瑞智[1] 张萍[1] 余波[1] 罗锐[1] 龚正林[1] 

机构地区：[1]安康市中心医院口腔科,725000

出　　处：《实用口腔医学杂志》2014年第4期526-529,共4页Journal of Practical Stomatology

摘　　要：目的:探讨富含半胱氨酸蛋白(cystein-rich 61,Cyr61)对人腺样囊性癌细胞系生物学特性的影响。方法:采用siRNA转染法抑制人腺样囊性癌细胞系ACC-LM和ACC2中Cyr61的表达;Western blotting检测Cyr61蛋白表达水平;MTT法检测Cyr61-siRNA对细胞增殖的影响;Transwell法检测Cyr61-siRNA对细胞侵袭的影响;Annexin V/PI双染法检测Cyr61-siRNA对细胞凋亡的影响。结果:Cyr61-siRNA能显著抑制ACC-LM和ACC2中Cyr61的表达;显著抑制ACC-LM和ACC2的增殖和侵袭,而对ACC-LM和ACC2细胞的凋亡则无显著影响。结论:Cyr61能促进人腺样囊性癌细胞的增殖和侵袭。Objective：To explore the effects of cysteine-rich 6 1 （Cyr6 1 ）on biological behavior of human adenoid cystic carcinoma ACC-LM and ACC2 cells.Methods：The chemically synthesized Cyr6 1-siRNA was transfected into ACC-LM and ACC2 cells.Cell proliferation was measured by the MTT method,the invasive ability was evaluated by Transwell chamber assay,and cell apoptosis was analyzed using flow cytometry by double staining with Annexin V and propidium iodide.Results：Cyr61-siRNA significantly down-regulated Cyr61 protein expression in ACC-LMand ACC2 cells.Cyr61-siRNA markedly inhibited the proliferation and invasion of the cells, however,there was no significant difference in cell apoptosis between Cyr6 1-siRNA and control groups.Conclusion：Cyr6 1 promote the proliferation and invasion of adenoid cystic cancer cells.

关 键 词：腺样囊性癌 Cry61 增殖 侵袭 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]