人类脐带间充质干细胞抑制肝内胆管癌细胞生长的机制  被引量:1

Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth

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作  者:刘娟[1] 韩国庆[1] 刘慧[1] 秦成勇[1] 

机构地区:[1]山东大学附属省立医院消化内科,山东济南250021

出  处:《山东大学学报(医学版)》2014年第8期1-5,共5页Journal of Shandong University:Health Sciences

基  金:国家自然科学基金(81250040);山东省自然科学基金(ZR2010HM046);中国博士后科学基金(2013M541926);山东省博士后创新专项(201302031)

摘  要:目的探讨人类脐带间充质干细胞(hUC-MSCs)对肝内胆管癌细胞生长的影响,深入了解其分子机制。方法采用hUC-MSCs条件培养基作用于肝内胆管癌HCCC-9810细胞,通过Transwell共培养实验、MTT比色实验、DNA断裂实验等方法检测细胞增殖和细胞凋亡,通过Western blotting及免疫荧光染色方法检测相关凋亡通路中蛋白质表达水平。结果间充质干细胞条件培养基对HCCC-9810细胞的增殖抑制表现出时间和剂量依赖性,且抑制作用是通过诱导细胞凋亡介导,增殖抑制率由12.87%升至50.98%,凋亡率由10.1%升至49.67%。p-Akt、p-GSK-3β表达下调。结论人类脐带间充质干细胞条件培养基可抑制肝内胆管癌细胞的生长,其分子机制与抑制PI3K/Akt信号通路有关。Objective To explore the effect of human umbilical cord mesenchymal stem cells on cholangiocarcinoma HCCC-9810 cells and its molecular mechanism. Methods HCCC-9810 cells were treated with conditioned media from umbilical cord mesenchymal stem cells. Cell proliferation and apoptosis were detected with co-culture systems,MTT assay and DNA fragmentation assay; the protein expressions were determined with Western blotting and immunoflorescence staining. Results Conditioned media from hUC-MSCs could inhibit proliferation and induce apoptosis of tumor cells in a dose- and time-dependent manner. The proliferation inhibition rate increased from 12. 87% to 50. 98%,whereas the apoptosis rate increased from 10. 1% to 49. 67%. The expressions of p-Akt and p-GSK-3β were down-regulated. Conclusion The hUC-MSCs conditioned media may suppress the growth of HCCC-9810 human cholangiocarcinoma cell line via PI3 K /Akt pathway.

关 键 词:脐带间充质干细胞 肝内胆管癌 AKT通路 

分 类 号:R735.7[医药卫生—肿瘤]

 

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