甲状旁腺激素对骨质疏松种植体骨结合的影响  

Effect of parathyroid hormone_(1-34) on bone healing around dental implants in ostoeporotic rats

作　　者：狄婧[1] 石晓婷[2] 李雪 林志勇[1] 孙哲[1] 邓凤英[1] 

机构地区：[1]山东大学附属省立医院口腔内科,山东济南250021 [2]天津市口腔医院儿童口腔科,天津300041 [3]山东医学高等专科学校口腔医学系,山东济南250014

出　　处：《山东大学学报（医学版）》2014年第8期22-26,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展基金(2010G0020226)

摘　　要：目的研究甲状旁腺激素(PTH1-34)对骨质疏松种植体骨结合的影响。方法 36只雌性Wistar大鼠随机分为假手术组、去势组和去势加PTH1-34治疗组,每组12只。假手术组接受假手术,去势组和去势加PTH1-34治疗组行双侧卵巢切除术。术后8周测量胫骨骨密度,证实骨质疏松状态。同时在3组大鼠右侧胫骨近干骺端分别植入纯钛螺纹柱状种植体,假手术组和去势组用生理盐水1mL/kg,去势加PTH1-34治疗组用PTH1-3420μg/kg,隔日腹腔注射1次,用药8周。种植术用药4、8周后处死各组半数大鼠,标本制作带种植体的骨组织磨片,行组织学观察及骨计量学测量。结果种植术后第4周,去势加PTH1-34治疗组骨小梁平均宽度、种植体骨结合率和结合骨板宽度均显著高于去势组(P<0.05),而去势加PTH1-34治疗组种植体骨结合率与假手术组相比,差异无统计学意义(P>0.05)。种植术后第8周,去势加PTH1-34治疗组松质骨区骨量、骨小梁平均宽度、种植体骨结合率和结合骨板宽度较去势组均有明显提高(P<0.05),与假手术组相比,差异无统计学意义(P>0.05)。结论骨质疏松对种植界面早期骨结合有一定的影响,间歇性、小剂量注射PTH1-34可有效促进骨形成,从而提高种植体骨结合的质量。Objective To investigate the effect of parathyroid hormone1-34（ PTH1-34） on bone healing around dental implants in ostoeporotic rats. Methods A total of 36 female Wistar rats were randomly divided into the sham-operation group,ovariectomized group and ovariectomized with PTH1-34 treatment group,with 12 in each group. Animals in the sham-operation group underwent sham-operation,while rats in ovariectomized group and ovariectomized with PTH1-34 treatment group received bilateral ovariectomy. Eight weeks after surgery,tibia of rats was examined for bone mineral density to verify osteoporosis status. At the same time,pure screw titanium implant was placed in the right tibia near metaphysis. Every other day for 8 weeks,normal saline 1 mL /kg was intraperitoneally injected in rats in sham-operation group and ovariectomized group,while PTH1-3420 μg /kg in rats in the ovariectomized with PTH1-34 treatment group. In week 4 and 8 after implantation surgery,half of the rats in each group were killed. Undecalcified sections were preparedand examined histologically and histomorphometrically. Results In week 4,the trabercular width（ TW）,implant bone contact rate（ IBCR）,combinded bone lamella width（ CBLW） of rats in ovariectomized with PTH1-34 treatment group were much higher than those of rats in ovariectomized group（ P〈0. 05）. In terms of IBCR,there was no difference between ovariectomized with PTH1-34 treatment group and sham-operation group（ P〈0. 05）. In week 8,trabercular area（ TA）,TW,IBCR and CBLW were significantly elevated in ovariectomized with PTH1-34 treatment group when compared with ovariectomized group（ P〈0. 05）. There was no difference between ovariectomized with PTH1-34 treatment group and sham-operation group in all parameters（ P〈0. 05）. Conclusion Osteoporosis impedes osseointegration in ovariectomized rats. Intermittent application of parathyroid hormone1-34 with small doses can promote bone formation effectively to improve the osseointegration quality of rats.

关 键 词：甲状旁腺激素 种植体 骨质疏松 骨结合 

分 类 号：R782.1[医药卫生—口腔医学]