贵州地区临床酵母菌种类组成和分布  

Composition and distribution of clinical yeasts in Guizhou region,China

作　　者：王梅竹[1] 曹煜[2] 赵亮[1] 徐小珊 张佩芳 吕倩[1] 白逢彦[4] 康颖倩[1] 

机构地区：[1]贵阳医学院微生物学教研室,贵州贵阳550004 [2]贵阳医学院附属医院皮肤性病科,贵州贵阳550004 [3]贵阳市白云区人民医院妇产科,贵州贵阳550014 [4]中国科学院微生物研究所真菌学国家重点实验室,北京100101

出　　处：《山东大学学报（医学版）》2014年第8期94-97,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金地区科学基金项目(31060006;31260029);贵州省科技厅社会发展科技攻关项目(黔科合SY字[2011]3017号);贵州省卫生厅科技项目(gzwkj2010-1-025);贵阳市科技局社会发展与民生计划(筑科合同[2011103]16号)

摘　　要：目的调查贵州地区临床常见病原真菌的种类及其分布特点。方法收集来源于贵州地区各种系统性疾病(深部组织来源)和外阴阴道念珠菌病(VVC)患者的临床酵母菌248株,根据CHROMagar培养基鉴定结果,进行基于ITS1区单链构象多态性(SSCP)图谱和序列分析的分子生物学菌种鉴定。结果分子生物学鉴定结果显示,收集的菌种主要来源于2个属10个种,其中优势属为Candida,优势种为C.albicans;分子生物学与CHROMagar培养基鉴定结果的符合率为91.53%;贵州地区深部组织与VVC来源的临床酵母菌的菌相分布具有显著差异。结论 ITS1区SSCP图谱结合序列测定分析在酵母菌种鉴定中是一种准确、可靠的方法。C.albicans仍为贵州地区的主要病原真菌,但其他酵母菌感染较以往报告呈增多趋势,特别是VVC来源的C.glabrata比例明显高于全国平均水平。Objectives To survey the species composition and distribution of clinical yeasts in Guizhou region,China.Methods Morphological observation,ITS1-SSCP patterning and sequencing were performed to identify 248 yeast strains from different clinical origins in Guizhou region,China. Results The identification result suggested that the isolated strains came from 2 genus and 10 species,in which the dominant genus and species were Candida and C. albicans,respectively. The molecular identification and CHROMagar testing coincidence rate was 91. 53%. The distribution of different sources of clinical yeasts was significantly different in Guizhou region. Conclusion The combination of ITS1-SSCP and sequencing analyses is accuracy and reliable when identifying the clinical yeasts with similar phenotypes. Although the most popular clinical yeast species is still C. albicans in local area,the increasing trend of non-albicans Candida species is noticed in this study,especially C. glabrata strains isolated from vulvovaginal andidiasis（ VVC） patients.

关 键 词：单链构象多态性 酵母菌 菌种鉴定 贵州 

分 类 号：R379[医药卫生—病原生物学]