miR-125a-5p抑制胶质瘤细胞增殖和糖酵解的初步研究  

miR-125a-5p inhibits proliferation and glycolysis of glioma cells: a preliminary study

作　　者：王宏瑜[1] 彭飞[1] 毕云科[1] 王开开 尹飞[1] 刘怀垒[1] 赵世光[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001

出　　处：《中华神经外科疾病研究杂志》2014年第4期313-316,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81272788)

摘　　要：目的研究微小核苷酸125a-5p(miR-125a-5p)在胶质母细胞瘤组织中的表达及其对胶质瘤细胞增殖和糖酵解的影响。方法定量反转录聚合酶连锁反应(qRT-PCR)检测miR-125a-5p在人源胶质母细胞瘤组织中的表达情况;利用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测miR-125a-5p对细胞活力的影响;分别利用三磷酸腺苷(ATP)和乳酸试剂盒检测miR-125a-5p对ATP和乳酸生成的影响。结果 miR-125a-5p在胶质母细胞瘤组织中的表达明显低于肿瘤周边组织;miR-125a-5p对胶质瘤细胞具有生长抑制作用;miR-125a-5p减少了胶质瘤细胞中ATP和乳酸的产生;miR-125a-5p和有氧糖酵解抑制剂2-脱氧葡萄糖(2-DG)具有协同抑制胶质瘤细胞增殖的作用。结论 miR-125a-5p抑制胶质瘤细胞的增殖和糖酵解,将来可能是潜在的胶质瘤治疗新靶点。Objective miR-125a-Sp expression in glioblastoma tissues and its effects on proliferation and glycolysis of glioma cells are studied. Methods Quantificational real-time polymerase chain reaction （qRT-PCR） was used to detect miR-125a-Sp expression in human glioblastoma tissues. Effect of miR-125a-Sp on cell viability was examined by 3- （ 4,5-dimethyhhiazol-2-yl ） -2, 5-diphenyltetrazolium bromide （MTY） assay. Production of ATP and lactate was assessed by ATP and lactate kit. Results miR-125a-Sp expression was significantly down-regulated in human glioblastoma tissues. Overexpression of miR-125a-Sp significantly reduced the cell viability and production of ATP and lactate in glioma cells. Moreover, miR-125a-Sp and 2-deoxyglucose （2-DG） had synergetic effects against glioma proliferation. Conclusion miR-125a-Sp inhibits proliferation and glycolysis of glioma cells, which may be a potential target for glioma theray in future.

关 键 词：微小核苷酸125a-5p 胶质瘤 增殖 糖酵解 2-脱氧葡萄糖 

分 类 号：R739.41[医药卫生—肿瘤]